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赣南脐橙致腐菌的分离鉴定及其对2-苯乙醇敏感性分析
Isolation and Identification of Citrus Pathogens from Navel Orange in Southern Jiangxi Province and Sensitivity Analysis to 2-Phenylethanol Alcohol
【摘要】 从采摘后贮存自然发病的赣南脐橙中分离出4种病原真菌,根据菌株形态学观察和rDNA-ITS序列分析进行鉴定,这4种病原真菌分别为意大利青霉(Penicillium italicum)、指状青霉(Penicillium digitatum)、柑橘链格孢(Alternaria citri)和芽枝状枝孢(Cladosporium cladosporioides)。生物保鲜剂2-苯乙醇(2-PE)可有效地抑制这4种病原真菌的孢子萌发和菌丝生长,但对不同致腐菌抑菌效果差异显著(P<0.05)。2-PE对芽枝状枝孢霉、指状青霉菌、柑橘链格孢和意大利青霉菌菌丝生长的最小抑菌浓度分别为1.4、1.8、2.2和2.4 g/L。
【Abstract】 Four citrus pathogens were isolated from rotten orange of Southern Jiangxi Province after harvesting. According to the observation of the morphological characteristics of fungi and the analysis of rDNA-ITS sequence, the four citrus pathogens were identified as Penicillium italicum, Penicillium digitatum, Alternaria citri and Cladosporium cladosporioides. The biopreservative 2-phenylethyl alcohol can effectively inhibit the spore germination and hyphae growth of the four citrus pathogens, but the antibacterial effects of different saprophytic fungi were significantly different(P<0.05). The minimum inhibitory concentrations for the hyphae growth of C. cladosporioides, P. digitatum, A. citri and P. italicum were 1.4, 1.8, 2.2 and 2.4 g/L, respectively.
【Key words】 Citrus pathogens; Morphological observation; Molecular identification; 2-Phenylethanol alcohol; Antibacterial;
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2020年07期
- 【分类号】S436.66
- 【被引频次】3
- 【下载频次】242