【摘要】 目的探讨长链非编码RNA-ROR(lncRNA-ROR)在慢性心力衰竭(CHF)患者血清中的表达水平及临床意义。方法收集25名正常人(对照组)及109例CHF患者,采用实时荧光定量PCR(real-time PCR)检测两组血清中lncRNA-ROR的表达,分析lncRNA-ROR的表达与N-末端脑钠肽前体(NT-proBNP)、左室舒张末期内径及左心室射血分数的相关性。结果对照组中lncRNA-ROR的表达水平相对较低,CHF患者中lncRNA-ROR的表达水平明显升高,差异有统计学意义(P <0.05)。相关分析发现lncRNA-ROR的表达水平与CHF心功能分级、左室舒张末期内径及NT-proBNP呈正相关,与左心室射血分数呈负相关(P <0.05)。结论lncRNA-ROR的表达水平与心力衰竭严重程度相关,有望成为早期诊断CHF的生物学标志物。更多还原

【Abstract】 Objective To explore the expression of long chain noncoding RNA-ROR(lncRNA-ROR) in the serum of the patients with chronic heart failure(CHF) and its clinical significance.Methods The expressions of lncRNA-ROR in the serum of 25 healthy subjects(control group) and in 109 patients with CHF were detected by Realtime PCR.The correlation between the expressions of lncRNA-ROR and NT-proBNP,left ventricular end-diastolic diameter and left ventricular ejection fraction was analyzed.Results The expression of lncRNA-ROR in the CHF patients was significantly higher than that in the control group(P < 0.05).The expression of lncRNA-ROR was positively correlated with cardiac functional grading of CHF,left ventricular end-diastolic diameter and NT-proBNP,and was negatively correlated with left ventricular ejection fraction(P < 0.05).Conclusion The expression of lncRNAROR is related to the severity of heart failure.lncRNA-ROR is expected to be a biomarker for early diagnosis of CHF.更多还原