【摘要】 以铺地刺蒴麻(Triumfetta procumbens)的嫩茎段为外植体,建立铺地刺蒴麻离体培养繁育和再生体系。结果表明,最适合诱导不定芽的培养基是添加0.8 mg·L-1 BA的MS培养基,平均每个外植体可以诱导出5.2个不定芽。当不定芽在增殖培养基上持续培养80 d后,在不定芽的叶片、叶柄和茎段上能够产生次生不定芽。不定芽在所有的生根培养基上均可生根。铺地刺蒴麻生根苗在生根培养基培育30 d后移栽到蛭石:河沙(体积比=1:1)的基质中, 1个月后成活率高达95.3%。更多还原

【Abstract】 The young stem sections of Triumfetta procumbens were used as explants, an efficient mass shoot proliferation and regeneration systems were established. The optimal medium for inducing adventitious shoot buds was MS medium supplemented with 0.8 mg·L-1 BA, which could induce an average of 5.2 adventitious shoot buds. As the stems sections were continuous cultured for 80 days, secondary shoot organogenesis could be induced on leaves and stem sections. All the rooting media could induce root formation. After 30 days of culture in rooting medium, the rooting seedlings were transplanted into the matrix with vermiculite: sand(1:1) for one month, the plantlets survival percentage was 95.3%.更多还原