【摘要】 对受酸性矿山废水污染的横石河上游到下游水体和沉积物中理化参数以及沉积物中真核微生物的多样性进行系统调查,使用斯皮尔曼相关性分析和典范对应分析(CCA)甄别真核微生物群落的主要环境胁迫因子.结果表明:横石河中真核微生物优势种群为真菌门(Fungi)(4.51%～86.69%)、绿藻门(Chlorophyta)(0.61%～77.36%)和纤毛虫门(Ciliophora)(0.81%～34.91%).沉积物中真核微生物群落Alpha多样性随酸性矿山废水污染梯度降低而逐渐升高.与原核微生物群落不同,酸性矿山废水环境沉积物中真核微生物群落结构的变化主要受硫酸根和电导率的影响.更多还原

【Abstract】 Analytical and molecular biology methods were integrated together to investigate the physicochemical factors in water and sediments of Hengshi River as well as the diversity of eukaryotic microorganism community in the sediments.Spearman correlation and canonical correspondence analysis(CCA) were further applied to identify the major factors affecting eukaryotic microorganism community.Results of 18 S rRNA gene sequencing indicated that Eukaryotic community of Hengshi River was dominated by Fungi(4.51%～86.69%),Chlorophyta(61%～77.36%) and Ciliophora(0.81%～34.91%).Both the abundance and evenness of eukaryotic communities in sediments significantly and gradually increased along the Hengshi River with decreasing contamination,which suggested that composition of eukaryotic microorganism community partly reflected changes in physicochemical conditions.Sulfate and electric conductivity of the sediments were the major abiotic factors altering the eukaryotic communities in the sediments of AMD impacted Hengshi River,which was different from the findings of prokaryotic microbial communities.更多还原