【摘要】 目的建立HPLC法测定雀舌茶Vaccinium dunalianum 6种主要化学成分6′-O-咖啡酰基熊果苷、熊果苷、robustaside A、对羟基苯甲酸、咖啡酸、咖啡酸甲酯的方法。方法采用Sun Fire^?C₁₈（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-0.1%磷酸水溶液,体积流量为1.0 m L/min,柱温为30℃,优化雀舌茶的提取方法,并考察方法的线性关系、精密度、稳定性、重复性。结果以蒸馏水为提取溶剂,超声提取20 min可以得到最高的提取率。在上述色谱条件下,6′-O-咖啡酰基熊果苷、熊果苷、robustaside A、对羟基苯甲酸、咖啡酸、咖啡酸甲酯有很好的分离效果,线性关系、稳定性、重复性较好。结论该方法操作简便,灵敏、准确,可以作为上述6种主要化学成分的检测方法。更多还原

【Abstract】 Objective To establish HPLC method for the determination of 6′-O-caffeoylarbutin(1), arbutin(2), robustaside A(3), p-hydroxybenzoic acid(4), caffeic acid(5) and caffeic acid methyl ester(6) of Queshe tea. Methods Using the SunFire? C18(250 mm×4.6 mm, 5 μm) chromatographic column, mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution, the flow rate at 1.0 mL/min, and column temperature at 30 ℃ to optimum the extraction method of Queshe tea and investigate the linearity, stability and repeatability of the method. Results With distilled water as the extraction solvent, the highest extraction rate can be obtained by ultrasonic extraction for 20 min. Under the above chromatographic conditions, 6′-O-caffeoylarbutin(1), arbutin(2), robustaside A(3), p-hydroxybenzoic acid(4), caffeic acid(5) and caffeic acid methyl ester(6) have good separation effect, and the experiment has good linearity, stability and repeatability. Conclusion The method is simple, sensitive and accurate, and can be used for the detection of the above six main chemical components.更多还原