【摘要】 为了观察与猪流行性腹泻病毒（PEDV） NSP12互作蛋白乳酸脱氢酶B （LDHB）的体外表达情况和小RNA干扰效果,构建了真核表达载体pcDNA4.0-LDHB-3*Flag,并在Vero细胞中过表达LDHB,发现在37 kDa处有大小相符的明显条带。设计的2条小干扰RNA能降低LLC-PK1细胞中LDHB的mRNA和蛋白质水平,经one-way ANOVA检验证明干扰实验组2^-ΔΔct的平均值显著低于对照组（P<0.05）。该结果为研究LDHB在PEDV感染LLC-PK1和Vero细胞过程中的作用提供了基础数据。更多还原

【Abstract】 In order to observe the in-vitro expression of LDHB interacting with PEDV NSP12 and the efficiency of the small interfering RNA,a eukaryotic expression vector named pcDNA4.0-LDHB-3*Flag was constructed to over-express LDHB in Vero cells,and a matching protein band was detected at 37 kDa.The mRNA and protein levels of LDHB were significantly down-regulated in LLC-PK1 cells after interfered by two small RNAs.One-way ANOVA test showed the mean of 2^-ΔΔct of experiment groups was substantially below the control groups（P<0.05）.This result provided a basis for the functional analysis of LDHB in the process of PEDV infecting LLC-PK1 and Vero cells.更多还原