【摘要】 角蛋白包括α角蛋白和β角蛋白两种类型.迄今α角蛋白被发现存在于所有的脊椎动物中,而β角蛋白仅存在于鸟类和爬行类表皮及其附属物中.本研究通过同源搜索比对鉴定了多疣壁虎(Gekko japonicus)的1个β角蛋白基因:G.japonicus claw keratin-like(LOC107121854),暂命名为ge-gprp-13,和1个α角蛋白基因:G.japonicus keratinocyte associated protein 2(KRTCAP2),暂命名为ge-krtcap2.利用荧光定量PCR方法比较分析了多疣壁虎ge-gprp-13和ge-krtcap2基因在其胚胎发育过程中的mRNA表达模式.研究结果表明,在多疣壁虎胚胎发育的不同时期,α角蛋白基因(ge-krtcap2)和β角蛋白基因(ge-gprp-13)的表达模式不同:在趾部发育的初期,α角蛋白基因先表达,而β角蛋白基因主要在趾部发育的后期进行表达,并且在趾部形成的整个过程中,β角蛋白基因的表达量远远高于α角蛋白基因的表达量.更多还原

【Abstract】 Reptile epidermis contains two types of keratins—α-keratins and β-keratins. Up to date,α-keratins are reported to be present in all vertebrates,while β-keratins are only found in birds and reptiles. In the present study,two keratin genes of Gekko japonicus were identified from the National Center of Biotechnology Information database,one β-keratin gene,G.japonicus claw keratin-like(LOC107121854)tentatively named ge-gprp-13 and one α-keratin gene,G.japonicus keratinocyte associated protein 2(KRTCAP2)tentatively named ge-krtcap2. The mRNA expression of keratin genes during embryonic development of the gecko was studied by using fluorescence quantitative real-time PCR. The results showed that the α-keratin gene(ge-krtcap2)and the β-keratin gene(ge-gprp-13)have different expression patterns in different stages of embryonic development of the gecko. In the early stage of toe development,the α-keratin gene is expressed first,while the β-keratin gene is mainly expressed in the later stage of toe development. And during the formation of the toe,the expression level of the β-keratin gene is much higher than the α-keratin gene.更多还原