【摘要】 利用快中子、返回式卫星搭载、重离子多种方式处理野生金莲花种子,研究其对金莲花生物性状及遗传物质的影响。结果表明:快中子在花部性状的作用更为显著,花形变异较多;而返回式卫星搭载的金莲花植株在叶型的变异较为明显;重离子辐照处理金莲花的分子标记结果显示,⁷Li250Gy（29.11%）多态性比率高于¹²C100Gy （21.95%）、⁷Li200Gy （21.87%）、¹²C150Gy （20.39%）;聚类分析表明,⁷Li 250Gy辐照产生的突变株与对照的遗传距离相对较远,其次是⁷Li200Gy、¹²C100Gy,最后是¹²C150Gy。本研究为金莲花优势新品种的选育提供了材料和新种质资源,也为诱变育种的应用和分子生物学水平上的研究提供了参考依据。更多还原

【Abstract】 The effects of fast neutron, returning satellite and heavy ion on the biological characters and genetic material of Trollies were studied. The results showed that the effect of fast neutrons on flower traits was more significant, and there were more flower shape variations. The variation of leaf shape in the returning satellites was more obvious. The molecular marker results of heavy ion irradiation treatment of Trollies showed that the polymorphism ratio of⁷Li250 Gy（29.11%）was higher than that of¹²C100 Gy（21.95%）,⁷Li200 Gy（21.87%） and¹²C150 Gy（20.39%）. Cluster analysis showed that the genetic distance between mutant strains irradiated by⁷Li 250 Gy and the control group was relatively far, followed by⁷Li200 Gy,¹²C100 Gy, and finally by¹²C150 Gy. This study provided materials and new germplasm resources for the selection and breeding of new varieties of Trollies, and also provided references for the application of mutagenesis breeding and the research on molecular biology level.更多还原