【摘要】 【目的】探究SiBAK1是否参与到BR信号通路以及细胞死亡调控通路中.【方法】克隆了谷子(Setaria italica)SiBAK1基因,并在拟南芥(Arabidopsis thaliana)油菜素内酯(Brassinosteroids,BRs)信号受体突变体bri1-5和细胞死亡突变体bak1-3/bkk1-1中过表达.【结果】SiBAK1定位于细胞质膜上,在植物的真叶和子叶的维管束及根、茎中均有表达;过表达SiBAK1能显著恢复BR受体突变体bri1-5植株矮小、叶片皱缩及下胚轴、根长缩短的表型,并且能够部分恢复bri1-5对外源BL(Brassinolide,BL)不敏感的表型;在bak1-3/bkk1-1背景下过表达SiBAK1能够显著抑制其细胞死亡表型.【结论】SiBAK1既参与到BR信号通路中,也参与到细胞死亡信号调控通路中.更多还原

【Abstract】 【Objective】 To investigate whether SiBAK1 is involved in the BR signaling pathway and the cell death regulatory pathway.【Method】 Setaria italica SiBAK1 gene was cloned and overexpressed in the Arabidopsis thaliana BR signal receptor mutant bri1-5 and the cell death mutant bak1-3/bkk1-1.【Result】 The results showed that SiBAK1 was localized on the cytoplasmic membrane and expressed in the vascular bundles of true leaves and cotyledons. It also expressed in roots and stems. Overexpression of SiBAK1 in bri1-5 could partially restore the BR receptor mutant phenotype,including short stature,leaf shrinkage and lower hypocotyl root length shortened in bri1-5 plants. It could partially refresh bri1-5 phenotype that was insensitive to exogenous BL. Overexpression of SiBAK1 in bak1-3 bkk1-1 could significantly inhibit plants cell death phenotype.【Conclusion】 Setaria italica SiBAK1 was involved in both the BR signaling pathway and the cell death signaling regulatory pathway.更多还原