【摘要】 目的建立人体毛发中西地那非成分的LC-MS/MS检验方法,并应用于实际案例。方法色谱柱为Thermo Hyperil Gold(50mm×2.1mm, 1.9μm),流动相为甲醇-5mM醋酸铵-0.1%甲酸,采用ESI源正离子多反应检测(MRM)扫描分析。结果西地那非的检出限为0.1ng/mL,定量限为0.2ng/mL,在0.2ng/mL～100ng/mL范围内线性良好,线性系数0.9999,回收率为86.7%～95.5%。结论该方法快速、灵敏、高效,可适用于实际案件人体毛发中西地那非成分的检验。更多还原

【Abstract】 Objective To establish a method for the determination of sildenafil in human hairs, and apply it to real cases. Methods The sample was analyzed on a Thermo Hyperil Gold（ 50 mm×2.1 mm, 1.9μm） column. The mobile phase consists of methanol-5 mM NH₄ AC-0.1%FA. LC-MS/MS was performed in ESI source with MRM mode.Results Working equation was constructed between peak area and concentration in the range of 0.2～100 ng/mL with the correlation coefficient larger than 0.999. The limit of detection of sildinafil was 0.1 ng/mL, and the average recovery rate was 86.7%～95.5%. Conclusion This proposed method is fast,sensitive and efficient, and suitable for the detection of sildenafil in human hairs in real cases.更多还原