【摘要】 以侧柏为试材,采用ELISA检测方法,研究了侧柏花芽分化前后球花着生位点鳞叶内源激素变化情况,分析了侧柏花芽分化发端时间,以期为控制侧柏花粉污染提供调控截止时间和调控参考药剂。结果表明:可能发育为雄球花或雌球花部位组织的6-BA、KT、GA₃、GA₄、GA₇和ABA的含量均在6月28日至7月5日极显著地升高,其中最低为6-BA达2.11倍,最高为GA₇达6.46倍;而此时鳞片叶中6-BA和ABA含量显著降低,KT、GA₄和GA₇含量极显著地升高,但升高量极显著地低于雄球花和雌球花部位,GA₃含量在此期间无显著差异。球花着生位点和鳞片叶中的ZT含量从6月初开始缓慢下降,至7月5日达最低值,而IAA含量从6月初开始缓慢上升,至7月5日达最高值。球花着生位点Zr含量从5月中旬至7月中旬保持不变,但鳞片叶中Zr含量于7月5日极显著地降低,为6月28日的0.67倍。推断侧柏花芽分化发端时间约为7月5日,高水平的6-BA、GA₃、GA₄和ABA有利于侧柏花芽分化、高水平的Zr不利于侧柏花芽分化。更多还原

【Abstract】 Platycladus orientalis was used as raw material,using ELISA method to measure the endogenous hormones contents during times of floral bud differentiation of P.orientalis,in order to provide the deadline and referring agents for controlling pollen pollution of P.orientalis.The results showed that the contents of 6-BA,KT,GA₃,GA₄,GA₇ and ABA increased significantly from June 28^th to July 5^th in tissues possibly developing into male or female cones,with the lowest 6-BA increasing 2.11 times and the highest GA₇ increasing 6.46 times.But,in tissues possibly developing into scale leaves,contents of 6-BA and ABA decreased significantly,contents of KT,GA₄ and GA₇ also increased significantly which markedly lower than contents increased in tissues possibly developing into male and female cones,and contents of GA₃ remained constant from June 28^th to July 5^th.The content of ZT decreased slowly from the beginning of June to the lowest level on July 5^th,while the content of IAA increased slowly from the beginning of June to the highest level on July 5^th in tissues possibly developing into cones and scales.The content of Zr remained unchanged from mid-May to mid-July in tissues possibly developing into cone,but the content decreased significantly on July 5^th,with 0.67 times of the content on June 28^th,in tissues possibly developing into scaly leaves.The changes in endogenous hormones inferred that the initiation time of flower bud differentiation of P.orientalis was around July 5.High levels of 6-BA,GA₃,GA₄ and ABA,but low levels of Zr,were beneficial to flower bud differentiation of P.orientalis.更多还原