【摘要】 目的:采用固相萃取法与高效液相色谱法合用,去除五味子宁神合剂中影响测定的杂质,建立五味子醇甲含量测定,对处方中的黄芪进行TLC定性鉴别。方法:采用C₁₈SPE小柱纯化样品,Waters C₁₈色谱柱（150 mm×3. 9 mm,5μm）;流动相:甲醇-水（55∶45）;流速:1. 0 ml·min^-1;检测波长:250 nm。TLC色谱定性鉴别采用硅胶G薄层板上,以氯仿-甲醇-水（15∶7∶2）为展开剂。结果:五味子醇甲在10～50μg·ml^-1范围内其线性关系良好（r=0. 999 4）,平均回收率99. 34%,RSD=1. 55%（n=9）。黄芪与对照品色谱相应的位置上,显相同的颜色。结论:优化后的方法简便、准确、快速、重复性好。更多还原

【Abstract】 Objective: To remove the impurities affecting the determination in Wuweizi Ningshen mixture,establish the determination method for schizandrin and qualitatively identify Astragali Radix by TLC. Methods: The sample was purified by using a C₁₈ SPE cartridge. The column was Waters C₁₈（150 mm × 3. 9 mm,5 μm）. The mobile phase was methanol-water（55 ∶ 45）. The flow rate was 1. 0 ml·min-1. The detection wavelength was 250 nm. The TLC was carried out on a silica gel G thin plate with chloroform-methanol-water（15 ∶ 7 ∶ 2） as the developing agent. Results: The linear range of schisandrin A was 10-50 μg·ml^-1（r = 0. 999 4）,the average recovery was 99. 34%,and the RSD was 1. 55%（ n = 9）. Astragalus and the reference standard showed the spot with the same color at the corresponding position in TLC. Conclusion: The optimized method is simple,accurate,fast and reproducible.更多还原