【摘要】 目的研究有机磷暴露接触对内质网的影响。方法大鼠持续60天氧乐果经口染毒,检测骨骼肌中的PERK,BiP,p-eIF2α,eIF2α,IRE1α的表达水平。结果染毒组的BiP,PERK和IRE1α的表达水平均高于Control组(P<0.05)。染毒组中BiP,PERK和IRE1α的表达水平中剂量组(MD)和高剂量组(HD)均高于低剂量组(LD)(P<0.05);与MD组相比,HD组中BiP,PERK和IRE1α的表达水平较高(P<0.05)。染毒组p-eIF2α/eIF2α水平均高于Control组(P<0.05)。与LD组和MD组相比,HD组中p-eIF2α/eIF2α的水平较高(P<0.05);LD组与MD组的peIF2α/eIF2α水平的差异不具有统计学意义(P>0.05)。结论氧乐果暴露接触能够引起大鼠体内产生内质网应激。更多还原

【Abstract】 Objective To explore the effects of organophosphate exposure on endoplasmic reticulum.Methods The rats were exposed to omethoate for 60 days through gastric injection,and the expression levels of PERK,BiP,p-eIF2α,eIF2α,IRE1α in muscles of rats from different groups were determined and compared.Results The expression of BiP,PERK and IRE1α in muscles of rats exposed to omethoate was significantly higher than that of normal rats(P<0.05).Compared with LD group,the expression of BiP,PERK and IRE1αin muscles of rats from MD and HD groups was significantly increased.The expression of BiP,PERK and IRE1αfrom HD group was significantly higher than that of MD group(P<0.05).The expression of p-eIF2α/eIF2α in muscles of rats exposed to omethoate was significantly higher than that of control group(P<0.05)and it was significantly higher of HD group than that of MD and LD groups(P<0.05).There was no significant difference in p-eIF2α/eIF2α between MD and LD group.Conclusion Exposure to omethoate can cause endoplasmic reticulum stress.更多还原