【摘要】 细菌生物被膜(bacterial biofilm,BBF)是在固—液和气—液表面上生长并封闭在胞外多糖基质中的细胞群,能调控细菌对环境胁迫的适应能力,提高细菌对紫外线(UV)胁迫等环境的抗逆性,是细菌的一种保护性生长方式。生防菌剂(biological control agents,BCAs)因其能以环境友好的方式长期控制病害虫等优点,一直是生物农药领域研究的热点。本文简介了细菌生物被膜及其应用,以苏云金芽胞杆菌(Bacillus thuringiensis,Bt)和枯草芽胞杆菌(Bacillus subtilis,Bs)为核心综述了BBF对生防活性的影响、生防细菌生物被膜形成、解离的表达、调控机制以及生物被膜调控基因的鉴定方法,并指出未来BBF在生防应用方面主要的研究方向,旨在为解决BCAs田间持效期较短等问题提供新的思路。更多还原

【Abstract】 Bacterial biofilm(BBF) is a cell population that grows on the surfaces of solid-liquid and gas-liquid and blocks in the exopolysaccharide matrix, and can regulate the ability of bacteria to adapt to environmental stress. It can also improve the resistance of bacteria to stress environments, including ultraviolet(UV) radiation. Herein, it is a protective growth of bacteria. Biological control agents(BCAs) have been the focus of researches in the field of biopesticide because of their long-term inhibition of diseases and control of insect pests in an environmentally friendly manner. This article describes the BBFs and their applications. Taking Bacillus thuringiensis(Bt) and Bacillus subtilis(Bs) as main examples, it also reviews the effects of biofilms on biocontrol activities, the regulatory mechanisms of the formation and dispersion of biofilm, the identification methods of biofilm-related genes, and points out the future research directions for biocontrol with BBF traits. We aim to provide new ideas for solving the application problems of BCAS such as relative short field duration.更多还原