【摘要】 为了解2016年山东地区H9N2亚型禽流感病毒的变异情况,选取分离鉴定的10株H9N2亚型禽流感病毒分离毒株,分别对其HA和NA基因进行序列测定和分子特性分析,并应用Mega 5.0软件绘制相应的基因氨基酸进化树,进行遗传进化分析。结果表明:10株H9N2分离毒的HA裂解位点氨基酸均为RSSR/GLF,具有典型低致病性AIV HA基因的特征;其第226位氨基酸均为亮氨酸(L),因此具有结合人流感受体的分子特性。同时这10株病毒的NA基因颈部均有9个核苷酸的缺失,且NA的潜在糖基化位点均不超过8个。进化树分析表明,2016年山东地区H9N2流行毒株HA和NA基因均属A/Chicken/Beijing/1/1994亚群,并且同属于近几年在中国鸡群中流行的G57分支。更多还原

【Abstract】 To evaluate the genetic variations of H9 N2 Avian influenza virus(AIV) circulating in 2016 in Shandong province, HA and NA genes of 10 isolates of H9 N2 AIV obtained in 2016 were sequenced for phylogenetic analysis using Mega 5.0 software. The results showed that the cleavage sites of their HA genes were all RSSR/GLF, showing the typical gene character of LPAIVs. The HA possessed leucine at 226 th amino acid position, indicating a human influenza receptor binding characteristic. The NA genes of these 10 isolates had a 9 bp deletion and their potential glycosylation sites were not more than 8. Phylogenetic analysis of HA and NA genes of these H9 N2 AIV isolates indicated that all of them belonged to the A/Chicken/Beijing/1/1994 subgroup and G57 lineage widely circulating among Chinese chicken populations in recent years.更多还原