【摘要】 目的分析无乳链球菌毒力基因的分布及其与耐药性和MLST的关系。方法收集深圳市南山区人民医院2013-2017年临床分离的136株无乳链球菌,采用BD细菌自动鉴定仪和质谱仪鉴定细菌种属;采用琼脂平板稀释法检测红霉素、克林霉素、四环素、左氧氟沙星、万古霉素、利奈唑胺、青霉素、氯霉素的MIC值;采用PCR方法检测毒力基因fbsA、fbsB、scpB、lmb、cylE、cfb、hylB、rib、bac、bca、cspA及管家基因。结果 136株无乳链球菌主要分离自尿液、血液,分别占29.41%和19.12%;无乳链球菌对红霉素、四环素及克林霉素的耐药率分别为52.21%、46.32%和45.59%;136株无乳链球菌以ST17(25株,占18.38%)、ST19(25株,占18.38%)和ST10(21株,占15.44%)为主,未检出Bac阳性菌株,毒力基因fbsB、cfb、hylB、Imb、cylE、cpsA、bca、scpB、fbsA、rib、cpsⅢ携带率均>50%。结论医院分离株无乳链球菌携带fbsB、cfb、hylB、Imb、cylE、cpsA、bca、scpB、fbsA、rib、cpsⅢ多种毒力因子,MLST分型以ST17、ST19为主,携带毒力基因菌株在抗菌药物敏感区段具有聚集性,未发现万古霉素耐药株。更多还原

【Abstract】 Objective To analyze the distribution of the virulence factors of Streptococcus agalactiae and their relationship to antimicrobial resistance and MLST. Methods A total of 136 strains of Streptococcus agalactiae isolated from 2013 to 2017 at Shenzhen Nanshan People’s Hospital were collected and identified by a BD Phoenix 100 automated microbial analyzer and mass spectrometer.The minimum inhibitory concentrations(MICs)of erythromycin,clindamycin,tetracycline,levofloxacin,vancomycin,linezolid,penicillin,and chloramphenicol were determined using agar dilution.A polymerase chain reaction(PCR)was used to detect virulence factors including fbsA,fbsB,scpB,Lmb,cylE,cfb,hylB,rib,bac,bca,and cspA and housekeeping genes. Results One hundred and thirty-six strains of Streptococcus agalactiae were mainly isolated from urine(32.0%)and amniotic fluid(20.0%).The resistance of Streptococcus agalactiae to erythromycin was 52.21%,its resistance to tetracycline was 46.32%,and its resistance to clindamycin was 45.59%.The 136 strains of Streptococcus agalactiae were mainly ST17(25 strains,18.38%),ST19(25 strains,18.38%)and ST10(21 strains,15.44%).The virulence factor Bac was not detected in any of the strains.The virulence factors fbsB,cfb,hylB,Imb,cylE,cpsA,bca,scpB,fbsA,rib,and cpsIII were all detected at a rate higher than 50%. Conclusion The virulence factors fbsB,cfb,hylB,Imb,cylE,cpsA,bca,scpB,fbsA,rib,and cpsIII were detected in hospital isolates of Streptococcus agalactiae.ST17 and ST19 were the major STs.Strains with those virulence factors were clustered in drug-sensitive segments,and none were resistant to vancomycin.更多还原