【摘要】 研究了光辐照和基于石墨相氮化碳（g-C₃N₄）光催化对二级出水中1株四环素和氨苄西林抗性多重耐药菌E.coli CGMCC 1.1595的灭活效果.结果表明,汞灯辐照功率（100/300/500W）和辐照强度越高,其灭活效率相对越高,在500W汞灯60min辐照条件下,紫外长波（UVA）-可见光（300⁵79nm）对该多重耐药菌的灭活率为0.41log;基于g-C₃N₄的光催化对其灭活率为1.31log.相比未加g-C₃N₄催化剂的光辐照灭菌,在UVA-可见光条件下g-C₃N₄对其灭活率的贡献为61%⁶9%;在可见光条件下g-C₃N₄对其灭活率的贡献达到60%⁷9%.在UVA-可见光g-C₃N₄光催化灭活E.coli CGMCC 1.1595反应体系中,活性氧自由基和电子空穴的活跃程度为:·OH>·O₂^->H₂O₂>h⁺>¹O₂,·OH为该光催化体系的主要活性物质,其次是·O₂^-和H₂O₂.更多还原

【Abstract】 The inactivation effects of multi-drug resistant bacterium E.coli CGMCC 1.1595against tetracycline and ampicillin from secondary effluent by light irradiation and photocatalysis with graphite carbon nitride（g-C₃N₄）were studied.The results showed that the higher irradiation power of the mercury lamp（100/300/500W）with higher irradiation intensity could lead to higher inactivation efficiency.Under the inactivation by 500W mercury lamp irradiation of 60min,the inactivation rate of E.coli CGMCC 1.1595was0.41log by ultraviolet A（UVA）-visible light（300⁵79nm）irradiation,and the inactivation rate was up to 1.31log by g-C₃N₄photocatalysis.The contribution of g-C₃N₄ to the UVA-visible light inactivation was 61%⁶9%compared to that without g-C₃N₄catalyst,while the contribution of g-C₃N₄ to the visible light inactivation was 60%⁷9%.The significance of the reactive oxygen species（ROS）and hole（h⁺）for the g-C₃N₄ photocatalytic inactivation of E.coli CGMCC 1.1595were also investigated,with the activity order as·OH>·O₂^->H₂O₂>h⁺>¹O₂.Hydroxyl radical（·OH）was a leading contributor to the irradiation,followed by·O₂^-and H₂O₂.更多还原