【摘要】 目的 :探讨MyD88依赖途径的相关基因TLR4、NF-κB与MyD88在膝骨关节炎(osteoarthritis,OA)不同病程下大鼠滑膜组织的表达特点及相关性。方法:将60只Wistar大鼠随机分为6组:空白组(N)、假手术组(F)和模型组[2周组(2W)、4周组(4W)、8周组(8W)、12周组(12W)],每组各10只。各模型组以Hulth法建立大鼠膝OA动物模型,空白组不予手术,假手术组仅打开关节腔。按上述设计时间收集样本,经总RNA的提取及反转录后,用Realtime PCR法对各组滑膜组织中TLR4、NF-κB及MyD88的相对表达量进行检测,分析其相关性。结果 :假手术组各基因的mRNA表达与空白组相比差异均无统计学意义((P>0.05);而各模型组较空白组各基因表达增强(P<0.05)。相关性分析显示:MyD88与TLR4及NF-κB的mRNA相关性系数分别为0.91和0.86,差异有统计学意义(P<0.05)。结论:MyD88与TLR4、NF-κB的表达均呈显著的正相关性,在通路上起重要的枢纽作用,可通过其表达预测通路上其他基因的表达情况,进一步明确膝OA病理机制。更多还原

【Abstract】 Objective:To investigate expression features and correlation of genes expression on MyD88-dependent signaling pathway in synovial membrane(SM) of progression of knee osteoarthritis(OA). Methods:Sixty Wistar rats were randomly divided into 6 groups,including blank group(N),false surgical group,model groups [2 weeks(2 W),4 weeks(4 W),8 weeks(8 W) and 12 weeks(12 W)],with 10 rats in each group. The models were established by using Hulth method. Control group was experienced no surgery,while false surgical group was only opened joint cavity and sutured. The SM samples was collected according to the time designed above. The relative expression quantity of MyD88,TLR4 and NF-κB was detected by Real-time PCR after the extraction of the total RNA and reverse transcription. The correlation analysis was obtained by SPSS.Results:There was no significant difference in each gene mRNA expression between false surgical and blank group(P>0.05),while enhanced expression was found in the model groups(P<0.05). The correlation index among MyD88,TLR4 and NF-κB was 0.91 and 0.86 respectively,and had significant difference among them. Conclusion:Positively relative among MyD88,TLR4 and NF-κB played main role in TLR4/NF-κB signal passway,and could predicate the expression of other genes in the passway. It also could further provide the basis for clarify the pathologic mechanism of knee OA.更多还原