【摘要】 目的对女性不孕不育患者进行生殖免疫性抗体[抗精子抗体(AsAb)、抗子宫内膜抗体(EMAb)、抗心磷脂抗体(AcAb)]的检测,并分析其临床意义。方法选取2015年1月-2017年1月于该院进行检查的不孕不育女性患者260例,将其作为不孕不育组;同时选取同时期于该院进行检查的健康女性260例,将其作为健康对照组。采用酶联免疫吸附试验(ELISA)法检测并比较两组女性血清中的AsAb、EMAb及AcAb抗体水平。结果在AsAb-T检测水平方面,不孕不育组总阳性率(65. 8%)与健康对照组总阳性率(5. 4%)相比明显提高(P<0. 05);在EMAb检测水平方面,不孕不育组总阳性率(31. 5%)与健康对照组总阳性率(5. 4%)相比明显提高(P<0. 05);在Ac Ab检测水平方面,不孕不育组总阳性率(23. 5%)与健康对照组总阳性率(3. 6%)相比明显提高(P<0. 05)。在AsAb、EMAb及AcAb抗体联合检测方面,不孕不育组女性两项同时阳性者47例,总阳性率为18. 1%,三项均为阳性者24例,总阳性率为9. 2%;而健康对照组无两项、三项同时阳性。结论引发女性不孕不育患者症状出现的重要因素主要包括AsAb、EMAb及AcAb这三类免疫性抗体,临床上在对不孕不育女性患者进行治疗时应加强全面检测免疫性抗体情况。更多还原

【Abstract】 Objective To detect reproductive immune antibodies in female infertile patients,including anti-sperm antibody( AsAb),endomethal antibody( EMAb),and anticardiolipin antibody( AcAb),analyze the clinical significance. Methods A total of 260 infertile women were selected from the Fourth People’s Hospital of Zigong from January 2015 to January 2017 as infertility group,and 260 healthy women were selected from the hospital during the same period as healthy control group. ELISA was used to detect and compare the levels of serum AsAb,EMAb,and AcAb in the two groups. Results The total positive rate of AsAb in infertility group was 65. 8%,which was statistically significantly higher than that in healthy control group( 5. 4%)( P<0. 05). The total positive rate of EMAb in infertility group was31. 5%,which was statistically significantly higher than that in healthy control group( 5. 4%)( P<0. 05). The total positive rate of AcAb in infertility group was 23. 5%,which was statistically significantly higher than that in healthy control group( 3. 6%)( P<0. 05). The positive rate of two indexes among AsAb,EMAb,and AcAb in infertility group was 18. 1%( 47 patients),the positive rate of combination of AsAb,EMAb,and AcAb in infertility group was 9. 2%( 24 patients). In healthy control group,the positive rates of two indexes among AsAb,EMAb,and AcAb and combination of AsAb,EMAb,and AcAb were 0. Conclusion AsAb,EMAb,and AcAb are important factors that cause symptoms of female infertile patients,reproductive immune antibodies should be detected comprehensively at the same time of treatment of infertility.更多还原