【摘要】 抗草甘膦大豆的安全性一直备受关注,对转G10-EPSPS基因抗草甘膦大豆SHZD32-01进行荒地生存竞争能力试验,以检验其是否具有杂草化风险。以转G10-EPSPS基因抗草甘膦大豆SHZD32-01、受体大豆中豆32、主栽品种皖豆28为材料,在上海交通大学农业与生物学院的转基因试验基地,采用地表撒播和常规方式播种,在2017年4月至7月分4次播种,每个处理重复4次,调查不同播种条件下大豆和杂草的生长情况,并对大豆及杂草群落的生长进行分析。结果发现,在地表撒播情况下,SHZD32-01与中豆32、皖豆28的存活率、株高、覆盖度和种植区域内杂草覆盖度基本无显著差异。在常规播种情况下,3种大豆种植区域内杂草发生情况无显著差异;4、5月播种的转基因大豆存活率弱于中豆32和皖豆28,且与中豆32差异显著;6月播种的3种大豆存活率均低于5%。在2种播种条件下,4月播种的转基因大豆繁育系数显著大于中豆32,5—7月播种的大豆繁育系数,三者之间差异不显著。综合分析上述指标,转基因大豆荒地生存竞争能力略弱于中豆32和皖豆28。同时所有试验小区8月杂草覆盖度均达到100%,转基因大豆SHZD32-01的荒地生存能力明显弱于杂草。在2种播种条件下,转基因大豆SHZD32-01与受体大豆、主栽品种以及杂草相比均没有竞争优势,没有转化为杂草的风险。更多还原

【Abstract】 The safety of glyphosate resistant soybeans has always been a concern. In this study,we tested the survival and competitiveness of transgenic glyphosate-resistant soybean SHZD32-01 with G10-EPSPS gene in wasteland to test whether it has the risk of becoming weedy. SHZD32-01,Zhongdou 32( receptor soybean of SHZD32-01) andmain cultivar Wandou 28 were used in this study. From April to July in 2017,all three varieties of soybeans were surface and conventionally sown and their growth as well as the weeds were analyzed at the agricultural experimental station of the School of Agriculture and Biology,Shanghai Jiao Tong University. Each treatment was repeated 4 times. There was no significant difference in survival rate,plant height,coverage and weeds coverage in growing area among SHZD32-01,Zhongdou32 and Wandou 28 under surface sowing. Under conventional sowing,there was no significant difference in weeds coverage,SHZD32-01 had lower survival rate than Zhongdou 32 and Wandou 28,and the differences between SHZD32-01 and Zhongdou32 were significant in most surveys. The survival rates of all types of soybeans sown in June were less than 5%. The breeding coefficient of SHZD32-01 sown in April was significant higher than that of Zhongdou32 under two different sowing conditions,but no such difference appeared when these soybeans were sown between May to July. Comprehensive analysis of the above indicators showed that the survival competitiveness of SHZD32-01 was slightly weaker than Zhongdou 32 and Wandou 28 in wasteland. Meanwhile,the coverage of weeds in all growing areas reached 100% in August,which meant the survival competitiveness of transgenic soybean was significantly lower than that of weeds. Transgenic soybean SHZD32-01 has no competitive advantage over the receptor cultivar,the commercial cultivar Wandou,and weeds under the two sowing conditions and does not have the risk of becoming weedy.更多还原