【摘要】 目的观察受体相互作用蛋白激酶3(receptor-interacting protein kinase 3,RIP3)在大鼠脊神经结扎模型中的表达,并探讨其是否参与大鼠神经病理性疼痛的发生。方法 40只大鼠随机分为手术组、生理盐水组、抑制剂组和假手术组,每组10只。手术组、生理盐水组和抑制剂组分别建立腰5脊神经结扎模型,假手术组只做手术,不结扎神经。生理盐水组、抑制剂组分别在建模30 min前鞘内注射生理盐水和GSK’872。记录各组大鼠的行为学改变与机械痛域,采用免疫组化和Western blot检测各组RIP3的表达水平,并通过ELISA法检测各组大鼠肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白介素1β(Interleukin-1β,IL-1β)含量。结果手术组、生理盐水组分别与抑制剂组、假手术组相比,均有明显行为学改变且机械痛域值明显下降(P<0.05),RIP3与TNF-α、IL-1β蛋白表达水平均显著上调(P<0.001);抑制剂组机械痛域值与RIP3含量均小于手术组和生理盐水组(P<0.05)。结论 RIP3在大鼠神经病理性疼痛模型中表达上调,可能参与神经病理性疼痛的发生。更多还原

【Abstract】 Objective To observe the expression of receptor-mediated protein kinase 3(RIP3)in a rat model of spinal nerve ligation and explore whether it is involved in the pathogenesis of neuropathic pain. Methods Rats were randomly divided into four groups:operation,saline,inhibitor and sham operation(n=10 rats per group). In the operation,saline,and inhibitor groups,a lumbar 5 spinal nerve ligation model was established. Animals in the sham operation group underwent surgery without nerve ligation. In the inhibitor group,GSK’872 was injected intrathecally at 30 min before model establishment;in the saline group,the same volume of saline was intrathecally injected. Behavior and mechanical allodynia were recorded for each group. RIP3 expression was analyzed using immuno-histochemistry and Western blotting;TNF-α and IL-1β levels were determined using ELISA. Results The operation and saline groups showed significant behavioral differences,significantly lower mechanical allodynia,as well as significantly higher levels of RIP3 protein,TNF-α and IL-1β than the sham operation group(P<0.05). The inhibitor group showed lower mechanical pain sensitivity and protein content than the operation and saline groups(P<0.05). Expression of RIP3,TNF-α and IL-1β negatively correlated with mechanical allodynia. Conclusions RIP3 is up-regulated in this animal model of neuropathic pain,so RIP3 may be involved in the development of neuropathic pain.更多还原