【摘要】 用流式细胞仪对骨髓细胞进行白血病细胞免疫分型,用RT-PCR方法主要检测了急性白血病样品中Hoxa1-13和Meis1基因的表达.结果表明在所有急性白血病患者中CD45和HLA-DR表达阳性,无T系抗原CD3表达,髓系抗原CD33和CD13阳性比例高(76%),B系抗原CD19阳性率为47%,而CD10和CD20阳性率为24%~29%.在急性白血病中Hoxa9和Meis1表达正相关,Hoxa6和Hoxa2表达大多呈现正相关,Hoxa1和Hoxa3在大多数样品中表达负相关.Hoxa3只在CD19阴性CD7阳性的急性白血病样品中表达.结果提示在急性白血病中Hoxa家族基因的表达异常,具有一定的表达相关性,并且免疫表型具有异质性,免疫抗原与Hoxa基因存在着一定的联系.更多还原

【Abstract】 We performed leukemia immunophenotyping of bone marrow cells with flow cytometry, and carried out RT-PCR to detect the expression of Hoxa1-13 and Meis1 in acute leukemia samples. Results showed that all acute leukemia samples expressed CD45 and HLA-DR, T cell antigen CD3 was not expressed, the positive rate of myeloid antigen CD33 and CD13 are 76%, B cell antigen CD19 is 47% while that of CD10 and CD20 is between 24 and 29%. In acute leukemia Hoxa9 positively correlated with Meis1, in most samples Hoxa6 and Hoxa13 showed a positive correlation, Hoxa1 and Hoxa3 are negatively related. Moreover, Hoxa3 was only expressed in acute leukemia samples with CD19-CD7+. These data suggested in acute leukemia Hoxa family genes are abnormally expressed, have certain correlation, and the immunophenotyping is heterogeneous, and immune antigens are closely linked with Hoxa gene expression.更多还原