【摘要】 目的:建立同时测定浓维磷糖浆中咖啡因、维生素B1和烟酸含量HPLC方法。方法:采用Ulti Mate XB-C18柱（4.6 mm×250 mm,5μm）色谱柱;以0.1%辛烷磺酸钠（含0.1%磷酸）为流动相A、乙腈为流动相B,梯度洗脱;流量1.0 m L·min^-1;柱温30℃;检测波长261 nm。结果:咖啡因、维生素B1和烟酸的线性范围分别为38.22³82.25μg·m L^-1（r=1.000 0）、7.28⁷2.75μg·m L^-1（r=0.999 5）和0.78⁷.84μg·m L^-1（r=1.000 0）;平均回收率（n=9）分别为99.3%、99.8%、98.6%,RSD分别为0.66%、1.14%、1.30%;170批样品含量测定结果为咖啡因0.28%⁰.33%、维生素B₁ 0.026%⁰.078%和烟酸0.004 6%⁰.0259%(g·m L^-1)。结论:所建方法经方法学验证,可用于浓维磷糖浆的质量控制。更多还原

【Abstract】 Objective: To develop an HPLC method for the determination of Caffeine,Vitamin B1 and Niacin in Concentrated Divitamins and Sodium Phosphate Syrup. Methods: The test was performed on Ulti Mate XB-C18（ 4. 6 mm × 250 mm,5 μm） column. The gradient elution was used with 0. 1% sodium octane sulfonate（ with 0. 1% phosphoric acid） solution as mobile phase A and acetonitrile as mobile phase B. The flow rate was set as 1. 0 m L·min-1,column temperature as 30 ℃ and detection wavelength as 261 nm. Results: The linearity ranges of Caffeine,Vitamin B1 and Niacin fell into 38. 22-382. 25 μg·m L^-1（ r =1. 000 0）,7. 28-72. 75 μg·m L^-1（ r =0. 999 5） and 0. 78-7. 84 μg·m L^-1（ r =1. 000 0） respectively. The average recoveries（ n =9） of Caffeine,Vitamin B1 and Niacin were99. 3%,99. 8% and 98. 6% with RSD as 0. 66%,1. 14% and 1. 30%,respectively. The contents of Caffeine,Vitamin B1,and Niacin in 170 batches of samples were 0. 28%-0. 33%,0. 026%-0. 078% and 0. 004 6%-0. 0259%( g·m L^-1). Conclusion: The methodology validation results show that the established method can be used for the quality control of Concentrated Divitamins and Sodium Phosphate Syrup.更多还原