【摘要】 通过密码子优化设计构建色氨酸合成酶基因工程菌,单因素以及响应面法研究色氨酸合成酶基因工程菌制备S-甲基-L-半胱氨酸。结果表明,温度39℃、pH 8.5、L-丝氨酸浓度360mmol/L,反应平衡时间20 h,S-甲基-L-半胱氨酸的质量浓度为40.49 g/L。更多还原

【Abstract】 Tryptophan synthase genetically engineered microorganism was constructed by codon optimization in Escherichia coli. Preparation of S-methyl-L-cysteine fromL-serine and methanthiol catalyzed by tryptophan synthase genetically engineered microorganism was investigated by response surface methodology and single-factor experiments. The optimal temperature and pH value were39 ℃ and 8.5 respectively. The optimal substrate concentration of L-serine was 360 mmol/L. Under the optimal conditions of tryptophan synthase genetically engineered microorganism,the content of S-methyl-L-cysteine was 40.49 g/L after 20 h.更多还原