【摘要】 目的:探讨蝮蛇毒血小板抑制因子(AHV-PI)减弱大鼠心肌缺血/再灌注(I/R)损伤作用及其可能作用机制。方法:雄性SD大鼠随机分为6组(n=6):正常对照组、I/R模型组、阳性对照组(银杏内酯BN52021,4 mg/kg)、AHV-PI低、中、高剂量组(0.05、0.1、0.2 mg/kg)。行大鼠心脏冠状动脉左前降支结扎30 min,再灌注120 min制备I/R模型,颈动脉取血并分离血小板,比浊法测定血小板聚集率;TTC染色检测心肌梗死程度;ELISA法测定血小板中血小板活化因子(PAF)含量。结果:与正常对照组相比,I/R组血小板聚集率上升(P<0.05),PAF含量增加(P<0.05),TTC染色梗死面积增加(P<0.01);AHV-PI中、高剂量组血小板聚集率降低,PAF释放减少,心肌缺血面积减少(P<0.05)。结论:AHV-PI可减轻大鼠心肌缺血再灌注损伤,其机制可能是通过抑制PAF的释放,进而抑制血小板聚集等实现的。更多还原

【Abstract】 Objective: To investigate the inhibiting effects of platelet inhibitor from Agkistrodon halys venom( AHV-PI) on the platelet activation in rats with myocardial ischemia reperfusion( I/R) injury and the potential inhibiting mechanisms. Methods: Male SD rats were randomized into 6 groups( n = 6 for each group),namely: normal control group,I/R group,positive control group( treated with inhibitor of platelet activating factor BN52021,4 mg/kg),AHV-PI group( treated with low dose: 0. 05 mg/kg; medium dose: 0. 1 mg/kg; and high dose: 0. 2 mg/kg,respectively). Rat models of myocardial I/R injury were developed by ligating the left anterior descending artery for 30 min,followed by 120 min reperfusion. Blood samples were obtained via carotid artery,and platelet was isolated. Turbidimetry was performed to measure the platelet aggregation rate,and TTC staining was used to detect the myocardial infarction size. ELISA was performed to determine the platelet activating factor. Results: Significantly increased platelet aggregation rate and PAF content as well as enlarged myocardial infarction size were seen in rats in I/R group compared to normal control group( P < 0. 05 or P < 0. 01). Marked decrease of platelet aggregation rate,reduced release of PAF and significantly improved infarction were observed in rats from AHV-PI group treated with medium and high dose of the snake venom( P < 0. 05). Conclusion: AHV-PI can effectively improve the myocardial I/R injury in model rats. The potential mechanisms may be associated with inhibited release of PAF release and platelet aggregation.更多还原