【摘要】 氨基酸透性酶(AAP)是植物中的氨基酸转运蛋白,在氨基酸转运过程中发挥着重要作用.以野生拟南芥(生态型col-0)为材料,利用PCR和DNA重组技术,克隆得到拟南芥中的氨基酸透性酶基因(At AAP6).该基因全长1,446,bp,构建At AAP6基因的植物表达载体pCAMBIA2300-At AAP6,并利用农杆菌介导的遗传转化方法在马铃薯品种"早大白"中异源过表达At AAP6基因.经过抗性筛选与植株再生,成功得到马铃薯转基因植株,并诱导得到转基因马铃薯微型薯.RT-PCR结果表明,At AAP6基因在转基因马铃薯中都能正常地转录表达.这一研究为进一步开展At AAP6基因功能验证,提高马铃薯块茎中的氨基酸以及贮藏蛋白的含量提供了研究基础.更多还原

【Abstract】 The amino acid permease(AAP)is the amino acid transporter in plants and plays an important role in the transport of amino acids.Amino acid permease gene(At AAP6)was cloned by PCR and recombinant DNA technologies using Arabidopsis thaliana(Columbia ecotype)as material.The full-length of the At AAP6 gene is 1,446,bp. The plant expression vector pCAMBIA2300-At AAP6 was constructed and transformed into virus-free plants of potato cultivar "Zaodabai" via Agrobacterium tumefaciens-mediated genetic transformation method.After resistance selection and plant regeneration,transgenic potato plants were successfully obtained.Meanwhile,transgenic potato microtubers were obtained successfully.The RT-PCR analysis proves that the At AAP6 gene could conduct normal transcription and expression in transgenic potato plants.This study provides the basis for further research on the functional verification of At AAP6 gene and the improvement of amino acids and proteins storage in potato tubers.更多还原