【摘要】 目的:探索敏咳汤对支气管哮喘豚鼠中炎性细胞(ECP)、炎性介质(NO)和细胞因子的影响。方法:选择清洁级封闭群幼龄雄性豚鼠50只,将其分为5组(哮喘模型组、中药大剂量组、中药小剂量组、地塞米松片对照组、正常对照组),采用卵白蛋白造模法。观察敏咳汤对哮喘豚鼠血清中炎性细胞(ECP)、炎性介质(IL-2、IL-3、IL-4、IL-13)和NO含量的影响。结果:敏咳汤可有降低血清中NO、ECP、IL-3、IL-13的含量、降低支气管肺泡灌洗液中IL-4的含量;提高血清IL-2的含量。结论:通过上述实验,可知敏咳汤从哮喘发病多个环节阻滞哮喘的发生。(1)敏咳汤可缩短哮喘发生症状的持续时间。(2)敏咳汤可升高IL-2的含量,恢复Th1/Th2类细胞因子免疫应答失衡,从而减少哮喘的发生。(3)敏咳汤可降低IL-4和IL-13的含量,减少IgE合成的细胞因子,阻滞哮喘的发生。(4)敏咳汤减少IL-3的含量,在支气管哮喘发病中,一则阻滞嗜碱、肥大细胞增殖和活性,二则减少嗜酸细胞活性。(5)敏咳汤可降低NO的含量,减少气道的炎症。这种机制在于NO能阻断嗜酸性粒细胞凋亡,从而间接加重或促进嗜酸性粒细胞炎症反应。同时作为信使的NO可抑制TH1细胞,促进TH2细胞的激活,致使激活炎性细胞的细胞因子IL-4生成过多,加重气道炎症反应。(6)敏咳汤可降低ECP的含量,减少哮喘季节性的发作,如花粉的诱发,减少气道的炎症反应。更多还原

【Abstract】 Objective:to explore the effects of Minke decoction on inflammatory cells(ECP),inflammatory mediators(NO)and cytokines in bronchial asthma guinea pigs.Methods:selected 50 young male guinea pigs of clean grade closed group and divided them into 5 groups(asthma model group,large dose group of traditional Chinese medicine,small dose group of traditional Chinese medicine,control group of dexamethasone and normal control group).Protein modeling.The ovalbumin moulding method was used.Experimental content:Effect of Minke decoction on inflammatory cells(ECP),inflammatory mediators(IL-2,IL-3,IL-4,IL-13)and NO in asthmatic guinea pigs.Results:Minke decoction can reduce the content of NO,ECP,IL-3 and IL-13 in serum,and decrease the content of IL-4 in bronchoalveolar lavage fluid,as well as increase the content of IL-2 in serum.Conclusions:Minke decoction blocks asthma from many aspects of asthma:(1) Min cough soup can shorten the duration of symptoms of asthma.(2) Minke decoction can increase the content of IL-2 and restore the imbalance of Th1/Th2 cytokine immune response to reduce the occurrence of asthma.(3) Minke decoction can decrease the content of IL4 and IL-13 and the synthesis of LgE cytokines to block the occurrence of asthma.(4) Minke decoction can decrease the content of IL-3 by attenuating basophil,mast cell and eosinophi proliferation ability and activity in the incidence of bronchial asthma.(5)Minke Decoction can decrease the content of N0 to reduce airway inflammation.The mechanism is that NO can block eosinophil apoptosis and indirectly aggravate or promote eosinophil inflammatory response.Meanwhile,NO,as a messenger,can respectively inhibit and promote the activation of TH1 and TH2 cells,resulting in the excessive production of inflammatory activating factor IL-4 to increase airway inflammation.(6) Minke decoction can decrease the content of ECP to reduce the onset of seasonal asthma(such as the induction by pollen)through reducing the airway inflammation.更多还原