【摘要】 旨在探讨植物蛋白质(PM)饲料基础上添加巨大芽孢杆菌对杂交鲟幼鱼组织抗氧化能力和血清生化指标的影响。以鱼粉(FM)组和PM组为对照组,试验Ⅰ、Ⅱ、Ⅲ组分别在PM组基础上添加105、106和107cfu/g本实验室筛选鉴定的巨大芽孢杆菌。试验选用初始平均体重为(12.87±0.65)g的杂交鲟幼鱼625尾,随机分成5组,每组设5个平行。试验结果表明:试验组血清总蛋白(TP)和球蛋白(GLB)含量显著高于PM组(P<0.05),尿素氮(BUN)含量和尿酸(UA)含量显著低于PM组(P<0.05),且与FM组相比差异均不显著(P>0.05);试验组组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性和还原型谷胱甘肽(GSH)含量显著高于PM组(P<0.05),黄嘌呤氧化酶(XOD)活性显著低于PM组(P<0.05)。综上,植物蛋白质饲料中添加适宜水平的巨大芽孢杆菌可提高杂交鲟幼鱼组织抗氧化能力、蛋白质利用率和机体免疫力。更多还原

【Abstract】 This experiment was conducted to study the effects of adding Bacillus megaterium to plant protein meal on tissue antioxidant capacity and serum biochemical indices of juvenile hybrid sturgeon. The fish in the 3 experimental groups were fed plant protein meal(group PM) with 105 cfu/g(group Ⅰ),106 cfu/g(group Ⅱ) and 107 cfu/g Bacillus megaterium(group Ⅲ),respectively,taking the fish meal(group FM) and group PM as control groups. A total of 625 juvenile hybrid sturgeon with an initial body weight of(12. 87 ±0. 65) g were randomly divided into 5 groups with 5 replicates and 25 fish per replicate. The results showed that the total protein(TP)content and globulin(GLB) content were higher in serum in the experimental groups compared with the group PM(P < 0. 05),the content of urea nitrogen(BUN) and the content of uric acid(UA) were lower compared with the group PM(P < 0. 05),and which had no significant difference with the group FM(P > 0. 05). The activity of total antioxidant activity(T-AOC) and superoxide dismutase(SOD),the content of glutathione(GSH) were higher in tissue in the experimental groups compared with that of the group PM(P <0. 05),the activity of Xanthine Oxidase(XOD) were lower compared with that of the group PM(P < 0. 05). In conclusion,the addition supplemental level of Bacillus megaterium to plant protein meal can improve the tissue antioxidant capacity,the protein utilization and immunity.更多还原