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表面活性剂对出芽短梗霉发酵生产聚苹果酸的影响

Effect of Surfactants on Poly Malic Acid Fermentation of Aureobasidium pullulans

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【作者】 赵廷彬陈畅殷海松孙爱友乔长晟

【Author】 ZHAO Ting-bin;CHEN Chang;YIN Hai-song;SUN Ai-you;QIAO Chang-sheng;College of Biotechnology,Tianjin University of Science & Technology;School of Bioengineering,Tianjin Modern Vocational Technology College;State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology;Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control;

【通讯作者】 乔长晟;

【机构】 天津科技大学生物工程学院天津现代职业技术学院生物工程学院华东理工大学生物反应工程国家重点实验室天津市微生物代谢与发酵过程控制技术工程中心

【摘要】 探究不同表面活性剂对出芽短梗霉CGMCC3337合成聚苹果酸的影响,并对最佳影响因子的作用机制做初步探究。结果发现,十二烷基硫酸钠(sodium dodecyl sulfate,SDS)和曲拉通X-100对聚苹果酸的合成有抑制作用;吐温80和吐温60在低浓度时有利于聚苹果酸的合成,最适添加量均为2 g/L;添加吐温80后,聚苹果酸的产量为31.36 g/L,较对照组提高30.5%。

【Abstract】 Influence of different surfactants on the synthesis of poly malic acid by Aureobasidium pullulans CGMCC3337 and the mechanism of action of the best influencing factors were studied. The results showed that sodium dodecyl sulfate(SDS)and Triton X-100 had an inhibitory effect on the synthesis of poly malic acid;Tween 80 and Tween 60 were favorable for the synthesis of poly malic acid at low concentrations,and the optimal amount was 2 g/L. After adding Tween 80 to the media,the yield of poly malic acid was 31.36 g/L,with an increase of 30.5 % when compared to the control.

【基金】 天津市科技支撑计划项目(17YFCZZC00310);天津市微生物代谢与发酵过程控制技术工程中心项目(ZXKF20180301)
  • 【文献出处】 食品研究与开发 ,Food Research and Development , 编辑部邮箱 ,2018年23期
  • 【分类号】TQ921;TQ423
  • 【被引频次】9
  • 【下载频次】212
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