【摘要】 采用多孔银丝表面增强拉曼（surface enhanced Raman scattering,SERS）基底,建立快速检测饮料中人工合成着色剂玫瑰红B的分析方法。饮料样品无需前处理,滤膜过滤后采用循环伏安法扫速25 m V/s制备的多孔银丝基底静置萃取8 min后即进行拉曼测试,结合固体标准品拉曼谱图和密度泛函理论（density functional theory,DFT）计算结果对玫瑰红B表面增强拉曼谱图中的特征峰进行了归属,以对玫瑰红B进行定性和定量测定。结果表明,1 277 cm^-1处的峰强度与玫瑰红B浓度的线性范围为5×10^-82.5×10^-6 mol/L,检出限为5×10^-9 mol/L。该方法原位萃取、测试时间只需9 min,可应用于饮料样品中玫瑰红B的快速检测,回收率在90.4%⁹2.3%,相对标准偏差小于5.2%。更多还原

【Abstract】 Rapidly screening method of man-made synthetic colorant Rhodamine B in food was established based on porous Ag fiber as surface enhanced Raman scattering（SERS） substrate. Without any sample pretreatment,silver fiber substrate synthesized with electrochemical assistance by Cyclic Voltammetry（CV）scanning at 25 m V/s was immersed in the membrane filtrated drink samples for 8 minutes without stirring. After that,the silver substrate was directly introduced for Raman analysis. The characteristic SERS spectrum peaks of Rhodamine B were attributed together with standard Raman and density functional theory（DFT） Raman spectra,followed by qualitative and quantitative analysis. The band at 1 277 cm^-1 was selected as the characteristic peak for quantitative determination,showing good linearity from 5 ×10^-8 to 2.5 ×10^-6 mol/L and detection limit of 5×10^-9 mol/L. It only takes 9 minutes for the in-situ extraction coupled with Raman test of the proposed method,which was successfully applied to fast identify of Rhodamine B in drink samples with recovery in the range of 90.4 %-92.3 % and good relative standard deviations（RSDs）less than 5.2 %.更多还原