【摘要】 为了探讨RANK/RANKL/OPG和WNT信号通路中6个基因10个单核苷酸多态性位点与中老年男性髋部骨强度的关联,征集788名广州地区40岁以上男性为研究对象,采用Mass Array检测基因型,双能X线吸收仪测定股骨颈骨密度(bone mineral density,BMD)、横截面积(cross-sectional area,CSA)、平均皮质厚度(average cortical thickness,ACT)、剖面模数(section modulus,SM)和抗屈曲率(buckling ratio,BR),以多重线性回归分析各位点与骨表型的关联。结果发现:OPG基因rs3134069的C等位基因与BMD(β=-0.017,P=0.024)和ACT(β=-0.004,P=0.040)负相关;LRP5基因rs3736228的T等位基因与低BMD、CSA、ACT(β=-0.050~-0.003,P<0.050)和高BR(β=0.242,P=0.043)相关,rs491347的G等位基因与低BMD相关(β=-0.012,P=0.046)。研究结果支持了OPG和LRP5基因与中国中老年男性人群髋部骨强度相关。更多还原

【Abstract】 The association of genes in the RANK/RANKL/OPG and WNT signaling pathways with hip bone strength was investigated among the middle-aged and elderly males. A total of 788 males aged over 40 years were included in the study. They were recruited from Guangzhou, China. Ten single nucleotide polymor-phisms in 6 genes were genotyped using Mass Array. Bone mineral density(BMD), cross-sectional area(CSA),average cortical thickness(ACT), section modulus(SM) and buckling ratio(BR) at the femoral neck were measured by dual-energy X-ray absorptiometry. The relationships between the genes and bone phenotypes were detected using multiple linear regression analysis. The results showed that the minor allele C of rs3134069 in OPG gene was negatively associated with BMD and ACT(β =-0.017, P=0.024 for BMD; β=-0.004, P=0.040 for ACT). In the LRP5 gene, the minor allele T of rs3736228 was related with the lower BMD, CSA, ACT(β=-0.050~-0.003, P<0.050) and higher BR(β=0.242, P=0.043), and the minor allele G of rs491347 was associated with the reduction of BMD(β=-0.012, P=0.046). This study supported that the OPG and LRP5 genes were associated with hip bone strength of the middle-aged and elderly males in Guangzhou.更多还原