【摘要】 目的探讨不同碱水解条件对4种稀有人参皂苷20（S）-Rh₁、20（S）-Rh₂、20（S）-PPt、20（S）-PPd含量的影响。方法应用高效液相色谱-质谱联用方法（HPLC-MS）初步推断人参茎叶总皂苷碱水解生成的人参皂苷结构,用高效液相色谱法（HPLC）测定不同碱水解反应时间、反应温度、氢氧化钠（NaOH）加入量对人参皂苷20（S）-Rh₁、20（S）-Rh₂、20（S）-PPt、20（S）-PPd含量的影响。结果碱水解40 min、反应温度分别为200℃和220℃、加入NaOH 2.0 g,有利于获得人参皂苷20（S）-Rh1和20（S）-Rh2;碱水解6 h、反应温度设置为240℃、加入NaOH 2.0 g,有利于获得人参皂苷20（S）-PPt和20（S）-PPd。结论人参皂苷20（S）-Rh₁、20（S）-Rh₂和人参皂苷20（S）-PPt、20（S）-PPd的含量受碱水解反应温度、NaOH加入量的影响趋势基本相同,但受碱水解反应时间的影响趋势不同。更多还原

【Abstract】 Objective To investigate the influence of different alkaline hydrolysis conditions on the contents of 4 rare ginsenosides including 20（ S）-Rh₁,20（ S）-Rh₂,20（ S）-PPt,and 20（ S）-PPd. Methods High performance liquid chromatography-mass spectrometry（ HPLC-MS） was applied to preliminarily infer the ginsenoside structure generated by alkaline hydrolysis of total saponin of ginseng stem and leaf. HPLC was adopted to determine the influence of different alkaline hydrolysis conditions,including reaction time,reaction temperature,amount of sodium hydroxide（ NaOH）,on the contents of ginsenosides 20（S）-Rh₁,20（ S）-Rh₂,20（S）-PPt and 20（S）-PPd. Results To obtain the high yield of ginsenoside 20（ S）-Rh₁ and 20（ S）-Rh₂,the condition was suggested as alkaline hydrolysis for 40 minutes,reaction temperature at 200 and 220 ℃ respectively and adding 2.0 g NaOH. To obtain the high yield of ginsenoside 20（ S）-PPt and 20（ S）-PPd,the condition was suggested as alkaline hydrolysis for 6 hours,reaction temperature at 240 ℃ and adding 2.0 g NaOH. Conclusion The influence trend of reaction temperature in alkaline hydrolysis and amount of Na OH on the contents of ginsenoside 20（ S）-Rh₁,20（ S）-Rh₂,20（ S）-PPt and 20（ S）-PPd was similar,while the influence trend of reaction time was different.更多还原