【摘要】 目的·观察青春期氰戊菊酯暴露对雄性大鼠睾丸组织氧化应激的影响。方法·将50只Sprague-Dawley(SD)雄性大鼠随机分为对照(玉米油)组、低剂量(0.02 mg/kg氰戊菊酯)组、中剂量(1 mg/kg氰戊菊酯)组、高剂量(50 mg/kg氰戊菊酯)组及干预(50 mg/kg氰戊菊酯+100 mg/kg N-乙酰基-L-半胱氨酸)组,每组10只,于4周龄开始连续染毒2个月。测定睾丸组织中丙二醛(malondialdehyde,MDA)含量、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和超氧化物歧化酶(superoxide dismutase,SOD)活力,并观察大鼠睾丸组织形态结构。结果·与对照组相比,高剂量组大鼠睾丸组织中MDA含量升高,体质量、GSH-Px和SOD活力均下降(均P<0.05);而干预组与高剂量组相比,MDA含量降低,GSH-Px活力升高(均P<0.05)。睾丸组织形态学观察发现,随着染毒剂量的增加,睾丸生精细胞排列疏松,细胞层数减少,曲细精管管腔内径增大。结论·青春期氰戊菊酯暴露可能导致雄性大鼠睾丸组织氧化损伤。更多还原

【Abstract】 Objective·To study the effects of fenvalerate exposure during puberty on oxidative stress in rat testis. Methods·Fifty male Sprague-Dawley(SD) rats were randomly divided into the control group(corn oil), low dose group(0.02 mg/kg fenvalerate), moderate dose group(1 mg/kg fenvalerate), high dose group(50 mg/kg fenvalerate) and intervention group(50 mg/kg fenvalerate+100 mg/kg N-acetyl-L-cysteine), ten rats for each group, for two months by gavage at four weeks of age. Malondialdehyde(MDA) content, activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in testis and testicular tissue morphology were detected. Results·Compared with the control group, the rat body weight and activities of GSH-Px and SOD in testis were significantly decreased in high dose group while MDA content was increased(all P<0.05). Compared with the high dose group, MDA content was decreased and GSH-Px activity was increased in the intervention group(both P<0.05). The results of testicular histology showed that with the increasing exposure dose, the spermatogenic cells were arranged loosely, the number of layers was decreased and the inner diameter of seminiferous tubules was increased. Conclusion·Exposure to fenvalerate during puberty may induce oxidative damage in testis tissue of male rats.更多还原