【摘要】 目的通过益气生津活血颗粒对链脲霉素(STZ)引发糖尿病大鼠心肌匀浆液中白介素-1(IL-1)、血浆内皮素(ET)、核因子κB(NF-κB)、肿瘤坏死因子α(TNF-α)以及血管内皮生长因子(VEGF)的影响来研究其对心肌微血管炎症损伤的防治作用机理。方法雄性SD大鼠,用链脲霉素加高脂饲料造糖尿病大鼠模型,成模后分别按分组灌胃给药,4周后处死动物,腹腔静脉取血,一部分测血糖,另一部分离心留血清,-80℃冷藏备用;然后,取心脏,研磨成液,然后分别按白介素-1、血浆内皮素、核因子κB、肿瘤坏死因子α说明书测含量;同时,分离心脏主动脉,进行免疫组化染色计算机定量分析,计算血管内皮生长因子值。结果模型组与用药组比较,白介素-1、血浆内皮素、核因子κB、肿瘤坏死因子α、血管内皮生长因子含量都有显著性差异。结论益气生津活血颗粒可降低链脲霉素引发糖尿病大鼠心肌中白介素-1、核因子κB、血浆内皮素、肿瘤坏死因子α、血管内皮生长因子含量,这或许是其对心肌微血管炎症损伤防治作用的机理。更多还原

【Abstract】 Objective To investigate the preventive and therapeutic mechanism of Yiqi Shengjin Huoxue Granules on inflammatory injury of myocardial microvascular injury of diabetic rats caused by streptozotocin(STZ) by measuring the level of interleukin-1(IL-1),plasma endothelin(ET),nuclear factor κB(NF-κB),tumor necrosis factor(TNF-α) and vascular endothelial growth factor(VEGF).Methods Diabetic rats induced by a single intravenous injection of streptozotocin and high-fat fodder were used as animal model of diabetes. After modeling,gavage according to the groups for four weeks,then the animals were executed.The blood was taken from abdominal vein,and one part of blood was used to measure the blood glucose,and the other was centrifuged and the blood plasma was stayed in-80 ℃.Then grinded the heart into liquid and measured IL-1,ET,NF-κB and TNF-α according to instructions. At the same time,separated the heart arteries,and quantitative analysis was carried out by immunohistochemical staining,then calculated the value of VEGF. Results Compared with the control group,there was difference in the content of IL-1,ET,NF-κB,TNF-α and VEGF.Conclusion Yiqi Shengjin Huoxue Granules can decrease the contents of IL-1,ET,NF-κB,TNF-α and VEGF in the myocardium of diabetic rats induced by STZ,and this may be the preventive and therapeutic mechanism of Yiqi Shengjin Huoxue Granules on inflammatory injury of myocardial microvascular injury.更多还原