【摘要】 从河北省围场县某养殖场患病死亡的犊牛脏器样品中分离到1株优势菌株,对其进行常规形态学观察、生化鉴定、16S r DNA基因序列测定、动物致病性试验及药敏试验。结果显示,分离菌株的生化鉴定特性与粪肠球菌的生化鉴定特性相符;分离菌株16Sr DNA基因序列与粪肠球菌的同源性为96. 2%;在系统发育进化树上分离菌株与粪肠球菌聚为一簇;分离菌株对小白鼠具有一定的致病性,并将其命名为EFC QHD-1;分离菌株EFC QHD-1对替米考星、复方新诺明、卡那霉素等耐药;对环丙沙星、氟苯尼考、阿莫西林等敏感。更多还原

【Abstract】 One dominant strain was isolated from a substantial organ sample of a diseased death calf in a farm in Weichang County,Hebei Province. It was subjected to routine morphological observation,biochemical identification,16 S r DNA gene sequencing,animal pathogenicity test and drug susceptibility test. The results showed that the biochemical identification results of the isolate were consistent with Enterococcus faecalis; the homology of the isolate with Enterococcus faecalis was 96. 2% as determined by the 16 S r DNA gene sequence;the isolates and Enterococcus faecalis clustered on the phylogenetic tree; the isolated strains had certain pathogenicity to mice,being named EFC QHD-1; the isolated strain EFC QHD-1 was resistant to compound sulfamethoxazole,tilmicosin and kanamycin,etc. and sensitive to ciprofloxacin,florfenicol and amoxicillin,etc.更多还原