【摘要】 目的探讨脂多糖通过NF-κb诱导食管鳞状上皮细胞CDX2的表达。方法采用免疫组化S-P法检测32例人正常食管组织和20例BE组织中NF-κb和CDX2的表达;体外培养正常食管鳞状上皮Het-1A细胞,采用不同浓度脂多糖(LPS)处理细胞,实时荧光定量PCR与蛋白印迹分析方法测定NF-κb和CDX2表达的变化。结果免疫组化检测发现:在20例BE组织中NF-κb阳性表达15例,阳性率75%,CDX2阳性表达16例,阳性率80%,与人正常食管鳞状上皮组织比较,BE组织中NF-κb、CDX2表达均增高(χ~2=20.734,P <0.05;χ~2=26.475,P <0.05)。Real-time PCR及Western Blot检测结果显示10ng/mL LPS组可轻微刺激食管鳞状上皮细胞NF-κb、CDX2的表达,与对照组无统计学意义。100ng/mL LPS和500ng/mL LPS组可明显增强NF-κb、CDX2的表达,且随浓度增强表达增强,差异有统计学意义(P <0.05)。结论脂多糖可能通过诱导食管鳞状上皮细胞NF-κb表达,上调BE标志性分子CDX2从而参与BE的形成。更多还原

【Abstract】 Objective To investigate the effect of lipopolysaccharide on the expression of CDX2 in esophageal squamous cells induced by NF-κb. Methods Immunohistochemical SP staining was applied to evaluate the levels of NF-κb and CDX2 in 32 samples of normal esophageal tissues and 20 samples of BE tissues. Normal esophageal squamous Het-1 A cells were cultured in vitro, and were manipulated with lipopolysaccharide at different concentration. The changes of expressions of NF-κb and CDX2 were examined by real time polymerase chain reaction and Western blot analysis. ResultsImmunohistochemistry revealed that: In 20 cases of BE, 15 cases had positive expression of NF-κb(75%),16 cases had positive expression of CDX2(80%). NF-κb and CDX2 was strongly expressed in the BE tissues compared with normal esophageal tissues, and its level was significantly higher in the BE(χ~2=20.734, P < 0.05; χ~2=26.475, P < 0.05). Real-time PCR and Western blot assay revealed that: 10 ng/mL LPS Group could slightly stimulate the expression of NF-κb and CDX2 in esophageal squamous cells, there was no significant difference between the two groups. The expression of NF-κb and CDX2 in 100 ng/mL LPS group and 500 ng/mL LPS group were significantly increased, and the expression increased with the concentration of LPS, the difference was statistically significant(P < 0.05). Conclusion Lipopolysaccharide may be involved in the formation of BE by inducing the expression of NF-κb in esophageal squamous epithelial cells and up-regulating the BE marker CDX2.更多还原