【摘要】 对纳米αFe₂O₃进行二巯基丁二酸表面修饰,采用透射电子显微镜（TEM）观察其形貌,利用振动样品磁强计（VSM）检测其磁学性能.配置含纳米αFe₂O₃浓度为10μg/m L的培养基培养牙髓干细胞,以不添加纳米αFe₂O₃的培养基为对照组.采用荧光显微镜观察纳米αFe₂O₃组与常规培养基组的细胞形态,以CCK-8检测细胞增殖情况,碱性磷酸酶（ALP）试剂盒检测细胞ALP活性,并进行茜素红染色以检验其矿化能力.结果表明,所用纳米αFe₂O₃为棒状,尺寸约10nm×90 nm,VSM结果显示其没有磁性.纳米αFe₂O₃培养基中牙髓干细胞的形态和增殖情况与常规培养基中情况没有差异,但是碱性磷酸酶活性和矿化能力则显著提高（p<0.05）.由此说明,纳米αFe₂O₃对牙髓干细胞的形态和增殖没有影响,但却会促进其向成骨方向分化.更多还原

【Abstract】 Nano αFe₂O₃ is surface modified with dimercaptosuccinic acid（ DM SA）. Its morphology is observed by transmission electronic microscopy（ TEM）,and its magnetic property is detected by vibration sample magnetometer（ VSM）. The media w ith 10 μg/m L nano αFe₂O₃ is used to culture dental pulp stem cells（ DPSCs） by using the media without nano αFe₂O₃ as the control. The morphology of DPSCs in the nano αFe₂O₃ group and the control group is detected by fluorescent stain. The cell proliferation is detected by CCK-8,and the cell alkaline phosphatase activity（ ALP） is measured by a ALP kit. Alizarin red staining（ ARS） is used to detect the mineral synthesis. The results show that nano αFe₂O₃ is spindle with about 10 nm × 90 nm in dimension. The VSM results prove that nanoαFe₂O₃ is nonmagnetic. No significant difference in cell morphology and proliferation betw een the nano αFe₂O₃ group and the normal media group is detected. However,both ALP activity and mineralization formation of cells are significantly increased（ p < 0. 05）. Therefore,nano αFe₂O₃ does not affect the cell morphology and proliferation,but promotes the osteogenic differentiation of DPSCs.更多还原