【摘要】 目的探讨同源异型盒基因B7(HOXB7)蛋白在结直肠癌中的表达及其与患者临床病理因素和预后的关系。方法回顾性分析结直肠癌患者87例,采用RT-PCR法和免疫组织化学法分别检测结直肠癌组织中HOXB7 mRNA和HOXB7蛋白的表达,分析其表达与患者临床病理参数和预后的相关性。结果 HOXB7 mRNA在结直肠癌组织中的相对表达量为(42.4±16.3),显著高于癌旁正常组织的(19.4±7.6),差异有统计学意义(P<0.05);HOXB7蛋白在结直肠癌组织中表达的阳性率为73.9%,显著高于癌旁正常组织的10.3%(P<0.05);HOXB7蛋白表达与患者TNM分期、淋巴结转移和远处转移有显著相关性(P<0.05),且HOXB7蛋白阳性表达的患者具有更差的预后。结论 HOXB7蛋白在结直肠癌组织中表达上调,其高表达与结直肠癌患者的临床病理因素和预后相关。更多还原

【Abstract】 Objective To investigate the expression of HOXB7 in colorectal cancer and its relationship with clinicopathological factors and prognosis.Methods Eighty-seven patients with colorectal cancer were retrospectively analyzed.The expression of HOXB7 mRNA and protein in colorectal cancer tissues was detected by RT-PCR and immunohistochemical methods.Their correlation with clinicopathological parameters and prognosis was analyzed.Results The relative expression level of HOXB7 mRNA in colorectal cancer tissue was(42.4±16.3),which was significantly higher than(19.4±7.6)in the paracancerous normal tissue(P<0.05).The positive expression rate of HOXB7 protein in colorectal cancer tissues was 73.9%,which was obviously higher than 10.3% in the paracancerous normal tissue(P<0.05);expression of HOXB7 protein was significantly correlated with the TNM stage,lymph node metastasis and distant metastasis(P<0.05),moreover the patients with HOXB7 positive expression had poorer prognosis.Conclusion HOXB7 protein expression is up-regulated in colorectal cancer tissue,and its high expression is correlated with the clinicopathological factors and prognosis in the patients with colorectal cancer.更多还原