【摘要】 目的:探讨细胞免疫在慢性荨麻疹发病中的作用,阐明慢性荨麻疹发生的免疫学机制。方法:选取慢性荨麻疹患者68例和健康对照者70名作为研究对象,根据不同并发症将慢性荨麻疹患者分为4个不同亚组,分别为自身免疫性疾病组11例(类风湿3例、红斑狼疮2例、甲状腺疾病4例、白癜风2例)、幽门螺旋杆菌感染组13例、乙肝和丙肝感染组10例、肿瘤组6例和无并发症组28例。采用流式细胞术检测各组研究对象外周血T细胞亚群表面标志CD3~+、CD4~+和CD8~+的表达情况,检测不同并发症慢性荨麻疹患者外周血T淋巴细胞亚群。结果:与对照组比较,慢性荨麻疹组患者外周血中CD4~+T淋巴细胞比例明显降低(P<0.05),CD8~+比例升高(P<0.05),CD4~+/CD8~+比值下降(P<0.05),CD3~+比例无明显变化(P>0.05);与病程≤12个月慢性荨麻疹患者比较,病程>12个月慢性荨麻疹患者外周血中CD4~+淋巴细胞比例明显降低(P<0.05),CD8~+比例升高(P<0.05),CD4~+/CD8~+比值下降(P<0.05),CD3~+比例无明显变化(P>0.05);与无并发症组患者比较,有并发症慢性荨麻疹组患者外周血中CD4~+和CD3~+比例亦降低(P<0.05),CD8~+比例升高(P<0.05),CD4~+/CD8~+比值下降(P<0.05)。结论:慢性荨麻疹患者体内存在细胞免疫功能异常,尤其CD4~+细胞的比例降低可能是疾病发生发展的机制之一。更多还原

【Abstract】 Objective:To study the effect of cellular immune in the pathogenesis of chronic urticaria,and to clarify the immunological mechanism of chronic urticaria.Methods:A total of 68 patients with chronic urticaria and70 normal controls were selected as the subjects.The patients with chronic urticaria were divided into four different subgroups according to the different complications;there were 11 patients in autoimmune disease group(3 case of rheumatism,2 case of lupus erythematosus,4 cases of thyroid diseases,2 cases vitiligo),13 patients in Helicobacter pylori infection group,10 patients in hepatitis B and hepatitis C group,and 6 patients in tumor group.The expressions of T cell surface markers CD3~+,CD4~+,and CD8~+in the peripheral blood were detected with flow cytometry.Furthermore,the T cell subsets in the peripheral blood of the patients with chronic urticaria with different complications were detected by flow cytometry.Results:Compared with control group,the ratio of CD4~+T cells of the patients in chronic urticaria group was significantly decreased(P<0.05),the ratio of CD8~+T cells was increased(P<0.05),and the ratio of CD4~+T cells/CD8~+T cells was decreased(P<0.05);but the ratio of CD3~+T cells had no changes(P >0.05).Compared with the patients with the disease duration less than12 months,the ratio of CD4~+T cells of the patients with the disease duration more than 12 months was significantly decreased(P<0.05),the ratio of CD8~+T cells was increased(P<0.05),and the ratio of CD4~+/CD8~+was decreased(P<0.05);but the ratio of CD3~+T had no changes(P>0.05).Compared with the patients without complications,the ratios of CD4~+T cells and CD3~+T cells of the patients with complications were decreased(P<0.05),the ratio of CD8~+T cells was increased(P<0.05),and the ratio of CD4~+/CD8~+was decreased(P<0.05).Conclusion:The unbalance of cellular immune exists in the patients with chronic urticaria,especially the decreasing of the ratio of CD4~+T cells in peripheral blood of the patients might be one of the mechanisms of pathogenesis of chronic urticaria.更多还原