【摘要】 目的探讨人参水提液(water extract of ginseng,WEG)对肿瘤相关巨噬细胞(tumor associated macrophages,TAMs)与肺癌A549细胞共培养体系肺癌A549细胞增殖、迁移及骨架蛋白F-actin表达等生物学行为影响。方法采用佛波酯(PMA)联合IL-4及IL-13诱导人急性白血病单核细胞株(THP-1)成为TAMs。以TAMs细胞上清与肺癌A549细胞共培养,模拟肿瘤微环境构建共培养模型。将细胞分为空白组(A549)、共培养组(TAMs+A549)、WEG高、中、低剂量组(TAMs+A549+WEG)。分别采用MTT法、实时细胞分析技术及高内涵细胞成像系统检测分析不同条件下A549细胞增殖、迁移和骨架蛋白F-actin表达情况。结果与空白组比较,共培养组A549细胞增殖明显增加,细胞迁移能力和细胞骨架面积均明显升高,差异有统计学意义(P<0.05);与共培养组比较,WEG高、中、低剂量组A549细胞增殖和迁移能力受抑制,细胞骨架面积和微丝条数减少,且呈剂量依赖性(P<0.05)。结论人参水提液在共培养体系中能有效抑制A549细胞增殖迁移能力,可能通过调节TAMs免疫活性而影响肿瘤细胞的生物学行为。更多还原

【Abstract】 Objective To study the effect of Water Extract of Ginseng(WEG) on the proliferation/metastasis of lung cancer A549 cells and the expression of F-actin in co-culture system of tumor associated macrophages(TAMs) and A549 cells. Methods Human acute leukemia mononuclear strain THP-1 was induced to become TAMs using Phorbol-12-myristate-13-acetate(PMA) combined IL-4 and IL-13. The supernant of TAMs and A549 cells were co-cultured. A co-culture model was set up by simulating microenvironment of lung cancer. Then cells were divided into the blank control group(A549), the co-culture group(A549 + TAMs), high, middle, and low dose WEG groups(TAMs + A549 + high, middle, and low dose WEG). The effects of WEG on the proliferation/metasta-sis of lung cancer A549 cells and the expression of F-actin under various conditions were detected using MTT method, Real time cell analysis(RTCA), and high content screening(HCS). Results Compared with the blank control group, the proliferation of A549 cells was obviously increased, cell migration was obviously elevated, and the area of cell skeleton was markedly enlarged in the TAMs + A549 group, with statistical difference(P < 0. 05).Compared with the TAMs + A549 group, the proliferation and migration of A549 cells were inhibited, the area of cell skeleton and the number of microfilaments were reduced dose-dependently(P < 0. 05). Conclusion WEG could effectively inhibit the proliferation and migration of A549 cells, which might be achieved by adjusting immunoactivities of TAMs, and further it affected biological behaviors of tumor cells.更多还原