【摘要】 对某酒企窖泥进行分离纯化培养,得到88株细菌纯培养物,通过16S rRNA基因序列比对和系统发育树分析,其中75株细菌被分为8个系统发育簇(clusterⅠ—Ⅷ),其最高相似菌分别为C.tyrobutyricum,C.butyricum,C.indolis,C.acetobutylicum,C.bifermentans,C.sporogenes,C.cadaveris和C.kluyveri.采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS)对8个代表性梭菌菌株的挥发性代谢物进行检测,共检测出75种挥发性物质,其中醇类23种、酸类20种、酯类14、醛类2种、酮类2种和其他挥发性物质14种.通过主成分分析发现,菌株RL1(C.tyrobutyricum)和EL7(C.butyricum)主要与乙酸和丁酸等化合物的形成有关;EL46(C.kluyveri)、RL20(C.acetobutylicum)和RL10(C.indolis)主要与己酸和己酸乙酯等化合物的形成有关;EL12(C.bifermentans)和RL23(C.sporogenes)主要与硫代醋酸S-甲酯、1-癸醇和正辛醇有关;而EL43(C.cadaveris)主要与多种酯类化合物的合成有关.更多还原

【Abstract】 A total of 88 pure cultures was obtained from the pit muds of one wine corporation following separation and purification culture. The 75 strains of bacteria were assigned into 8 phylogenetic clusters( clusterⅠ-Ⅷ) based on the analysis of 16 S rRNA gene sequences and phylogenetic tree. In them,the most similar bacteria were C. tyrobutyricum,C. butyricum,C. indolis,C. acetobutylicum,C. bifermentans,C. sporogenes,C. cadaveris and C. kluyveri,respectively. The volatile metabolites of 8 representative Clostridium spp.were analyzed using headspace solid-phase microextraction( HS-SPME) method followed by gas chromatography-mass spectrometry( GC-MS). A total of 75 volatile metabolites was detected,including 23 alcohols,20 acids,14 esters,2 aldehydes,2 ketones and other 14 volatile substances. The principal component analysis revealed that the representative strain RL1( C. tyrobutyricum) and EL7( C. butyricum) were mainly related to the generation of acetic acid and butyric acid,and EL46( C. kluyveri),RL20( C. acetobutylicum) and RL10( C. indolis) mainly contributed to the formation of caproic acid and ethyl hexanoate,and EL12( C.bifermentans) and RL23( C. sporogenes) were mainly associated with the S-methyl acetate,1-Decyl alcohol and octanol,and EL43( C. cadaveris) was mainly correlated to the biosynthesis of various esters.更多还原