【摘要】 目的:建立金黄连口服液的质量提高标准。方法:采用TLC法对金银花、黄芩、连翘中的绿原酸、黄芩苷、连翘苷进行定性鉴别;采用HPLC法定量测定金银花中绿原酸的含量,色谱柱:Promosil C₁₈色谱柱（250 mm×4.6 mm,5μm,Agela technologies公司）;流动相:乙腈-0.4%磷酸（10∶90）;检测波长为327 nm;流速:1.0 ml·min^-1;进样量:10μl,柱温:25℃。结果:薄层鉴别斑点清晰,在与对照药材相同的位置上,显相同颜色的荧光斑点,阴性样品无干扰;绿原酸含量在5.325¹70.400μg·m^-1之间,线性良好（r=0.999 8）,平均回收率为97.95%,RSD为2.04%（n=6）。结论:本方法结果可靠,操作简便,可作为金黄连口服液的质量控制方法。更多还原

【Abstract】 Objective:To improve the quality standard for Jinhuanglian oral liquid.Methods:The three herbs in the preparation,Honeysuckle,Scutellaria Baicalensis Georgir and St John’s Wort,were identified by TLC qualitatively.The content of chlorogenic acid in Lonicerae Japonicae Flos was determined by HPLC.The chromatographic separation was carried out on a Promosil C₁₈（250 mm×4.6 mm,5μm）column at 25℃.The mobile phase consisted of acetonitrile-0.4%phosphoric acid（10∶90）with gradient elution at a flow rate of 1.0 ml·min^-1,and the injection volume was 10μl.The detection wavelength was 327 nm.Results:The spots in TLC were clear without any interference.The linear range of chlorogenic acid was 5.325-170.400μg·ml^-1（r=0.999 8）.The average recovery was 97.95%and the RSD was 2.04%（n=6）.Conclusion:The method is reliable and easy to operate,which can be used as the quality control method for Jinhuanglian oral liquid.更多还原