【摘要】 为了研究肠炎沙门菌(SE)对TLR4信号通路调节雏鸡空肠和回肠组织中多聚免疫球蛋白受体(pIgR)的影响,本试验以7日龄海兰褐雏鸡的空肠和回肠组织为主要研究对象,通过口服SE的方式感染雏鸡,于感染后的1,3,7和14d,检测雏鸡的空肠和回肠组织中TLR4信号通路下游因子MyD88、TRAF6、NF-B和pIgR mRNA表达。结果表明:雏鸡感染SE后,TLR4信号通路被激活,且空肠、回肠组织中pIgR mRNA的表达量升高(P<0.01),同时上调空肠和回肠组织中pIgR的蛋白水平。证实SE能够激活空肠和回肠黏膜细胞表面的TLR4信号通路并上调pIgR的表达,进而增强雏鸡的肠黏膜免疫。更多还原

【Abstract】 To observe the effect of pIgR on Salmonella Enteritidis induced inflammation in jejunum and ileum in Chicken,7day Hyline chickens were taken orally with Salmonella Enteritidis(SE)and killed after 1d,3d,7dand 14 d.The mRNA expression of Toll-like receptor 4(TLR4),MyD88,TRAF6,NF-B and pIgR was detected by real-time RT-PCR and pIgR protein level was detected by Western blot.The results showed that TLR4 signaling pathway was activated and mRNA level of the pIgR in jejunum and ileum was enhanced(P<0.01)and protein level of the pIgR in jejunum and ileum was up-regulated by SE.The study proved that TLR4 signal pathway on mucosal cell surface of jejunum and ileum was activated and expression of pIgR was up-regulated and gut mucosal immunity of chicken was strenghtened.更多还原