【摘要】 目的:探讨LPS诱导的炎症小鼠子宫巨噬细胞迁移和功能活性的调节因素。方法:将150只雌性昆明种小鼠随机分为对照组(A组)、LPS模型组(B组)、MCP-1阻断组(C组),于末次注射后的1、3、6、12、24 h取子宫。运用免疫组织化学方法检测CD14~+巨噬细胞数量与CD14表达的变化,ELISA方法检测TNF-α、MCP-1的表达量。结果:(1)与A组相比,B组内膜、肌层、外膜的CD14~+巨噬细胞数目及CD14表达量在各时间点均极显著增加(P<0.01),C组内膜和肌层在1、3、6 h时恢复至正常水平;与B组相比,C组外膜在1、3、6 h时以及C组的内膜和肌层在各时间点时CD14~+巨噬细胞数目及CD14表达量均极显著减少(P<0.01)。(2)与A组相比,B组在各时间点以及C组在12、24 h时TNF-α和MCP-1的含量极显著增多(P<0.01);与B组相比,C组各时间点的TNF-α和MCP-1的含量极显著减少(P<0.01)。结论:LPS诱导的小鼠子宫炎症反应中巨噬细胞的迁移及CD14、TNF-α等关键分子的表达均受MCP-1的调节。更多还原

【Abstract】 Objective: To explore the adjustment factors to the migration and functional activity on macrophages in the uterine of inflammatory mice induced by LPS. Methods: 150 Kunming female mouse were divided into control group( group A),LPS model group( group B),MCP-1 blocking-up group( group C),the mice uterines were extracted separately at hour 1,3,6,12,24. The number of CD14~+macrophages and the expression of CD14 macrophages were detected by Immunohistochemistry,ELISA detects the expression of TNF-α and MCP-1. Results:(1)Compared with group A,the number of CD14~+macrophages and the expression of CD14 in endometrium,myometrium,perimrtrium of group B were highly significantly increased( P<0. 01) at every time points,the endometrium,myometrium of group C were closely to normal level at 1,3,6 h; compared with group B,the number of CD14~+macrophages and the expression of CD14 were highly significantly decreased( P<0. 01) at 1,3,6 h of perimrtrium of group C and every time points of endometrium,myometrium of group C.(2)Compared with group A,the content of TNF-α and MCP-1 were highly significantly increased( P<0. 01) at every time points of group B and 12,24 h of group C; compared with group B,the content of TNF-α and MCP-1 of group C were highly significantly decreased( P<0. 01) at every time points. Conclusion: The migration of macrophages and the expression of CD14 and TNF-α in the uterine of inflammatory mice induced by LPS were regulated by MCP-1.更多还原