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共轭亚油酸-精氨酸的制备及其抗氧化活性研究
Preparation and Antioxidant Activity of Conjugated Linoleic Acid-Arginine Complex
【摘要】 等摩尔的共轭亚油酸(CLA)和精氨酸(Arg)通过溶解、混合、浓缩和冷冻干燥等步骤制得水溶性的共轭亚油酸-精氨酸(CLA-Arg)复合物,探究了CLA-Arg复合物对DPPH自由基、ABTS自由基的清除能力和对卵黄脂蛋白过氧化的抑制作用,并通过Rancimat法测定不同抗氧化剂对大豆油氧化诱导时间的影响。结果表明:CLA-Arg复合物对DPPH自由基(IC502.76 mg/mL)和ABTS自由基(IC501.43 mg/mL)均有良好的清除效果;在浓度为1.0 mg/mL时,其对卵黄脂蛋白过氧化体系的抑制率达到41.8%;在添加量为0.1%时,使得氧化诱导时间延长到1.79 h且高于BHT,从而CLA-Arg具有良好的抗氧化活性。
【Abstract】 Water-soluble conjugated linoleic acid-arginine(CLA-Arg) complex had been prepared by dissolving,mixing,concentration and freeze drying from same mole of CLA and Arg.The antioxidant activity of CLA-Arg was studied by measuring its scavenging effects on DPPH radical and ABTS radical,inhibition effect on liposome peroxide and influence on oxidation induction time by Rancimat.The results showed that CLA-Arg had strong scavenging capacity on DPPH radical(IC50 2.76 mg/mL) and ABTS radical(IC50 1.43 mg/mL);the inhibition rate of liposome peroxide system was 41.8% when the concentration was 1.0 mg/mL;the oxidation induction time extended to 1.79 h and was higher than BHT when the content was 0.1%.CLA-Arg had a significant antioxidant activity.
【Key words】 water-soluble; conjugated linoleic acid-arginine(CLA-Arg); free radical; antioxidant activity;
- 【文献出处】 中国粮油学报 ,Journal of the Chinese Cereals and Oils Association , 编辑部邮箱 ,2017年06期
- 【分类号】TS218
- 【下载频次】132