【摘要】 目的探讨Ras,C-myc癌基因异常表达与慢性皮肤溃疡癌变的关系。方法选取10例Marjolin溃疡鳞状细胞癌组织标本。选取10例慢性皮肤溃疡伴假上皮瘤样增生组织标本,选取10例原发性皮肤鳞状细胞癌组织标本,选取5例正常皮肤组织标本。对标本中Ras,C-myc癌基因表达进行免疫组化分析。结果 Marjolin溃疡中鳞状细胞癌组织中,散在癌细胞Ras,C-myc阳性表达,为核染色,而正常组织以及原发性皮肤鳞状细胞癌组织两癌基因均阴性表达,另外,癌基因Ras,C-myc在慢性皮肤溃疡伴假上皮瘤样组织中不典型增生的上皮脚部位,也呈阴性表达。结论 Ras,C-myc的表达异常与慢性皮肤溃疡癌变成鳞状细胞癌有关。更多还原

【Abstract】 Objective To explore the relationship between the abnormal overexpression of Ras and C-myc in chronic ulcers of the skin and the pathogenesis of squamous cell carcinoma(SCC) in Marjolin′s ulcer. Methods Ten tissue specimens of SCC in Marjolin′s ulcer, ten tissue specimens of chronic skin ulcer accompanied with pseudoepitheliomatous hyperplasia, ten tissue specimens of primary SCC, and five tissue specimens of normal skin were collected. The expression of Ras and C-myc in these specimens was detected by immunohistochemistry. Results Both Ras and C-myc were overexpressed in the tissues of SCC in Marjolin′s ulcer. The nuclear staining of Ras and C-myc was positive in some carcinoma cells. However, they were negatively expressed in normal skin tissue and primary SCC cells. They were also not expressed in the epithelial cells with atypical hyperplasia in chronic skin ulcer accompanied with pseudoepitheliomatous hyperplasia. Conclusion The abnormal expression of Ras and C-myc is closely related to the carcinogenesis in chronic ulcer.更多还原