【摘要】 目的探讨干扰素-γCA重复基因多态性与成人呼吸道合胞病毒感染的相关性,为成人呼吸道合胞病毒感染的治疗提供参考。方法选择2014年12月-2015年12月医院收治180例毛细支气管炎患者,根据检查结果分为RSV感染毛细支气管炎（RSV组）78例和非RSV感染毛细支气管炎（非RSV组）102例,另选择同期在医院体检的60例健康人群为对照组,采用聚合酶链反应及毛细管电泳检测IFN-γCA基因多态性。结果 RSV组患者中(CA₁₂)+/(CA₁₂)+、(CA₁₂)+/(CA₁₂)-基因型PSI评分显著低于(CA₁₂)-/(CA₁₂)-基因型,IFN-γ浓度显著高于(CA₁₂)-/(CA₁₂)-基因型（P<0.05）。RSV组(CA₁₂)+/(CA₁₂)+基因型显著低于非RSV组和对照组,(CA₁₂)-/(CA₁₂)-基因型显著高于非RSV组和对照组（P<0.05）,非RSV组与对照组组间各基因型比较差异无统计学意义。结论干扰素-γCA重复基因多态性与成人呼吸道合胞病毒感染毛细支气管炎的发生、发展密切相关,当IFN-γCA基因重复12次时,IFN-γ表达量降低,机体对病毒的清除能力减弱,临床症状加重。对RSV感染患者进行优质护理对加快患者康复具有重要意义。更多还原

【Abstract】 OBJECTIVE To explore the correlation between interferon-γ（IFN-γ）CA gene polymorphism and adult respiratory syncytial virus infection so as to provide guidance for treatment of adult respiratory syncytial virus infection.METHODS A total of 180 patients with capillary bronchitis who were treated in the hospital from Dec 2014 to Dec 2015 were enrolled in the study,78 patients who were infected with RSV were assigned as the RSV group,and 102 patients who were not infected with RSV were set as the non-RSV group,meanwhile,60 healthy people who received physical examination were chosen as the control group.The IFN-γCA gene polymorphism was determined by using polymerase-chain-reaction（PCR）and capillary electrophoresis.RESULTS In the RSV group,the PSI score of the patients who had(CA₁₂)+/(CA₁₂)+,(CA₁₂)+/(CA₁₂)-genotypes was significantly lower than that of the patients who had(CA₁₂)-/(CA₁₂)-genotype;the IFN-γconcentration of the patients who had(CA₁₂)+/(CA₁₂)+,(CA₁₂)+/(CA₁₂)-genotypes was significantly higher than that of the patients who had(CA₁₂)-/(CA₁₂)-genotype（P<0.05）.The(CA₁₂)+/(CA₁₂)+ genotype of the RSV group was significantly lower than that of the non-RSV group and the control group,while the(CA₁₂)-/(CA₁₂)-genotype of the RSV group was significantly higher than that of the non-RSV group and the control group（P<0.05）;there was no significant difference in the genotypes between the non-RSV group and the control group.CONCLUSION The interferon-γCA gene polymorphism is closely associated with the occurrence and development of capillary bronchitis induced by adult respiratory syncytial virus.When the IFN-γCA gene repeated for 12 times,the level of expression of IFN-γdeclines,the body′s ability to clear viruses weakens,and the clinical symptoms aggravate.It is of great significance for the rehabilitation of the patients with RSV infection to conduct high-quality nursing.更多还原