【摘要】 目的探讨人参皂苷Rg1热裂解产物（HPPRg1）对H₂₂荷瘤小鼠的抗肿瘤作用以及机制。方法建立ICR小鼠H₂₂皮下移植瘤小鼠模型,观察HPPRg1的抗肿瘤作用。首先将H₂₂荷瘤小鼠随机分为空白组（normal）、模型组（model）、阳性药环磷酰胺组(CTX,30 mg·kg^-1)、HPPRg1低、中和高给药组(10、20和40 mg·kg^-1)。通过计算抑瘤率、脏器指数和肿瘤组织切片染色来观察HPPRg1的抗肿瘤作用。结果与模型组比较,HPPRg1 3个剂量组均能剂量依赖性抑制H₂₂肿瘤的增长（r=0.99,P<0.05）,抑瘤率分别为35.7%,42.9%和47.3%;此外,HPPRg1能够不同程度升高小鼠血清中肿瘤坏死因子（TNF）-α,干扰素（IFN）-γ和白细胞介素（IL）-2水平,病理组织染色表明,HPPRg1能够明显促进肿瘤细胞凋亡和坏死;其主要通过抑制瘤重细胞增殖及促凋亡而发挥抗肿瘤作用。结论 HPPRg1对H₂₂移植瘤具有明显的抑制作用,其机制可能与促进肿瘤细胞凋亡及提高机体免疫力有关。更多还原

【Abstract】 OBJECTIVE To investigate the antitumor effect and molecular mechanism of ginsenoside Rg1 pyrolysis products （HPPRg1） on H₂₂ tumor bearing mice. METHODS To establish tumor model of transplanting H₂₂ tumor-bearing mice and observe the anti-tumor effects of HPPRg1,H₂₂ tumor-bearing mice were randomly divided into groups of control,model,cyclophosphamide (CTX,30 mg·kg^-1),low dosage of HPPRg1 (HPPRg1-L,10 mg·kg^-1),middle dosage of HPPRg1 (HPPRg1-H,20 mg·kg^-1)and high dosage of HPPRg1 (HPPRg1-H,40 mg·kg^-1) groups,respectively. Through evaluating inhibition rates of tumors,organ indices,and levels of TNF-α,IFN-γ and IL-2 to observe the anti-tumor effect of HPPRg1. In addition,H&E and Hoechst 33258 straining were used to observe the apoptosis of H₂₂ tumor cell. RESULTS Compared with the model group,the three dose groups of HPPRg1 can inhibit tumor proliferation. Mainly through the inhibition of tumor cell proliferation and pro-apoptosis to exert anti-tumor effect. CONCLUSION HPPRg1 has a significantly inhibitory effect on H₂₂ tumor-bearing mice,the mechanism may related to promote apoptosis of tumor cells and improve immunity.更多还原