【摘要】 目的探讨CD34、基质金属蛋白酶(MMP-9)、血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)在骨巨细胞瘤(GCTB)组织中的表达及其临床意义,为GCTB的诊断与治疗提供临床依据。方法将2010年1月至2017年1月收治的80例GCTB手术患者纳入研究,采用免疫组化染色法检测GCTB组织中CD34、MMP-9、VEGF、PCNA的表达水平,分析其与GCTB患者年龄、性别、肿瘤大小、Jaffe分级以及预后之间的相关性。结果CD34、MMP-9、VEGF、PCNA的阳性表达与GCTB患者年龄、性别无明显相关性(P>0.05),与肿瘤大小、Jaffe分级、预后有显著相关性(P<0.05)。CD34与MMP-9、VEGF、PCNA的表达呈正相关(r=0.311,0.424,0.401;P=0.015,0.025,0.025)。结论 CD34、MMP-9、VEGF、PCNA在GCTB的发生发展中有重要作用,检测其表达水平对GCTB的诊断和预后具有一定的临床意义。更多还原

【Abstract】 Objective To explore the expression and clinical significance of CD34, matrix metalloproteinase(MMP)-9, vascular endothelial growth factor(VEGF), proliferating cell nuclear antigen(PCNA) in giant cell tumor of bone(GCTB), so as to provide clinical basis for the diagnosis and treatment of GCTB. Methods A total of 80 patients with GCTB treated from January 2010 to January 2017 were enrolled in this study. The expression of CD34, MMP-9, VEGF and PCNA in these patients was assayed by immunohistochemistry staining,and relations of expression of CD34, MMP-9, VEGF, PCNA with age, gender, tumor size, Jaffe grading and prognosis were analyzed. Results Positive expression of CD34, MMP-9, VEGF and PCNA showed no significant correlation with age or gender for GCTB patients, while levels of CD34, MMP-9, VEGF and PCNA were related to tumor size, Jaffe grading and prognosis; CD34 levels showed positive correlation to the expression of MMP-9,VEGF and PCNA(r = 0.311, 0.424, 0.401; P = 0.015, 0.025, 0.025). Conclusion CD34, MMP-9, VEGF, PCNA play important roles in the development and progression of GCTB, examination of expressions of these cytokines have certain clinical significances for the diagnosis and prognosis of GCTB.更多还原