【摘要】 本文从技术原理、研究进展及在动物疫病检测中的应用三个方面对环介导等温扩增(LAMP)技术进行了阐述。与普通PCR、荧光定量PCR相比,LAMP技术具有不需要精密仪器、肉眼即可判定、操作简单、所用时间短、灵敏度高等优点,在水生和陆生动物的细菌病、病毒病、寄生虫病的检测中得到广泛应用。LAMP技术与其他技术的联合应用以及LAMP检测仪器的出现,更加促进了LAMP技术的发展和应用。更多还原

【Abstract】 In this article,the Loop-mediated isothermal amplification(LAMP) technology was introduced in three aspects:technical principle,research progress and its application in animal diseases detection.Compared with the general PCR and fluorogenie quantitative PCR,the LAMP method has many advantages,including no need of precision instruments,judging results only by naked eyes,easy operation,costing less time and high sensitivity.Based on these advantages,the LAMP has been widely applied to the detection of bacterial,viral and parasitic diseases of aquatic and terrestrial animals.The development and application of LAMP are further facilitated by the combined application between LAMP and other techniques,as well as the emergence of the LAMP detecting instrument.更多还原